diff --git a/README.md b/README.md
index a92020df90698733cf1ac36770cc0298b4b8945f..3fd845d5e26ffeb092d7096292c88cdd4a6c56ee 100644
--- a/README.md
+++ b/README.md
@@ -103,4 +103,20 @@ echo "~/03DataPLANT_gitlab/samplearc_rnaseq/" > workflows/_arc_local_wd
 
 
 # replace 
-# runs/ by runs/'$run_name'/
\ No newline at end of file
+# runs/ by runs/'$run_name'/
+
+
+# PKG_PATH="http://cdn.NetBSD.org/pub/pkgsrc/packages/NetBSD/$(uname -p)/$(uname -r|cut -f '1 2' -d.)/All/"
+# export PKG_PATH
+# pkg_add pkgin
+
+
+
+# git clone https://github.com/pachterlab/kallisto.git
+# cd kallisto
+# mkdir build
+# cd build
+# cmake .. -DUSE_HDF5=ON
+
+-- Build files have been written to: /Users/dominikbrilhaus/kallisto/build
+
diff --git a/runs/run1/01_kallisto_results/DB_097/abundance.tsv b/runs/kallisto_sleuth/run1/01_kallisto_results/DB_097/abundance.tsv
similarity index 100%
rename from runs/run1/01_kallisto_results/DB_097/abundance.tsv
rename to runs/kallisto_sleuth/run1/01_kallisto_results/DB_097/abundance.tsv
diff --git a/runs/run1/01_kallisto_results/DB_097/run_info.json b/runs/kallisto_sleuth/run1/01_kallisto_results/DB_097/run_info.json
similarity index 100%
rename from runs/run1/01_kallisto_results/DB_097/run_info.json
rename to runs/kallisto_sleuth/run1/01_kallisto_results/DB_097/run_info.json
diff --git a/runs/run1/01_kallisto_results/DB_099/abundance.tsv b/runs/kallisto_sleuth/run1/01_kallisto_results/DB_099/abundance.tsv
similarity index 100%
rename from runs/run1/01_kallisto_results/DB_099/abundance.tsv
rename to runs/kallisto_sleuth/run1/01_kallisto_results/DB_099/abundance.tsv
diff --git a/runs/run1/01_kallisto_results/DB_099/run_info.json b/runs/kallisto_sleuth/run1/01_kallisto_results/DB_099/run_info.json
similarity index 100%
rename from runs/run1/01_kallisto_results/DB_099/run_info.json
rename to runs/kallisto_sleuth/run1/01_kallisto_results/DB_099/run_info.json
diff --git a/runs/run1/01_kallisto_results/DB_103/abundance.tsv b/runs/kallisto_sleuth/run1/01_kallisto_results/DB_103/abundance.tsv
similarity index 100%
rename from runs/run1/01_kallisto_results/DB_103/abundance.tsv
rename to runs/kallisto_sleuth/run1/01_kallisto_results/DB_103/abundance.tsv
diff --git a/runs/run1/01_kallisto_results/DB_103/run_info.json b/runs/kallisto_sleuth/run1/01_kallisto_results/DB_103/run_info.json
similarity index 100%
rename from runs/run1/01_kallisto_results/DB_103/run_info.json
rename to runs/kallisto_sleuth/run1/01_kallisto_results/DB_103/run_info.json
diff --git a/runs/run1/01_kallisto_results/DB_161/abundance.tsv b/runs/kallisto_sleuth/run1/01_kallisto_results/DB_161/abundance.tsv
similarity index 100%
rename from runs/run1/01_kallisto_results/DB_161/abundance.tsv
rename to runs/kallisto_sleuth/run1/01_kallisto_results/DB_161/abundance.tsv
diff --git a/runs/run1/01_kallisto_results/DB_161/run_info.json b/runs/kallisto_sleuth/run1/01_kallisto_results/DB_161/run_info.json
similarity index 100%
rename from runs/run1/01_kallisto_results/DB_161/run_info.json
rename to runs/kallisto_sleuth/run1/01_kallisto_results/DB_161/run_info.json
diff --git a/runs/run1/01_kallisto_results/DB_163/abundance.tsv b/runs/kallisto_sleuth/run1/01_kallisto_results/DB_163/abundance.tsv
similarity index 100%
rename from runs/run1/01_kallisto_results/DB_163/abundance.tsv
rename to runs/kallisto_sleuth/run1/01_kallisto_results/DB_163/abundance.tsv
diff --git a/runs/run1/01_kallisto_results/DB_163/run_info.json b/runs/kallisto_sleuth/run1/01_kallisto_results/DB_163/run_info.json
similarity index 100%
rename from runs/run1/01_kallisto_results/DB_163/run_info.json
rename to runs/kallisto_sleuth/run1/01_kallisto_results/DB_163/run_info.json
diff --git a/runs/run1/01_kallisto_results/DB_165/abundance.tsv b/runs/kallisto_sleuth/run1/01_kallisto_results/DB_165/abundance.tsv
similarity index 100%
rename from runs/run1/01_kallisto_results/DB_165/abundance.tsv
rename to runs/kallisto_sleuth/run1/01_kallisto_results/DB_165/abundance.tsv
diff --git a/runs/run1/01_kallisto_results/DB_165/run_info.json b/runs/kallisto_sleuth/run1/01_kallisto_results/DB_165/run_info.json
similarity index 100%
rename from runs/run1/01_kallisto_results/DB_165/run_info.json
rename to runs/kallisto_sleuth/run1/01_kallisto_results/DB_165/run_info.json
diff --git a/runs/run1/03_kallisto_mappingStats.csv b/runs/kallisto_sleuth/run1/03_kallisto_mappingStats.csv
similarity index 100%
rename from runs/run1/03_kallisto_mappingStats.csv
rename to runs/kallisto_sleuth/run1/03_kallisto_mappingStats.csv
diff --git a/runs/run1/03_kallisto_tpmMatrix.csv b/runs/kallisto_sleuth/run1/03_kallisto_tpmMatrix.csv
similarity index 100%
rename from runs/run1/03_kallisto_tpmMatrix.csv
rename to runs/kallisto_sleuth/run1/03_kallisto_tpmMatrix.csv
diff --git a/runs/run1/04_sleuth_dge.csv b/runs/kallisto_sleuth/run1/04_sleuth_dge.csv
similarity index 100%
rename from runs/run1/04_sleuth_dge.csv
rename to runs/kallisto_sleuth/run1/04_sleuth_dge.csv
diff --git a/runs/run1/05_shinyPrep.RData b/runs/kallisto_sleuth/run1/05_shinyPrep.RData
similarity index 100%
rename from runs/run1/05_shinyPrep.RData
rename to runs/kallisto_sleuth/run1/05_shinyPrep.RData
diff --git a/runs/run1/isa.run.xlsx b/runs/kallisto_sleuth/run1/isa.run.xlsx
similarity index 100%
rename from runs/run1/isa.run.xlsx
rename to runs/kallisto_sleuth/run1/isa.run.xlsx
diff --git a/runs/kallisto_sleuth/run2/03_kallisto_mappingStats.csv b/runs/kallisto_sleuth/run2/03_kallisto_mappingStats.csv
new file mode 100644
index 0000000000000000000000000000000000000000..21b8d2b5c3158bf2102012cb0216197f58087c98
--- /dev/null
+++ b/runs/kallisto_sleuth/run2/03_kallisto_mappingStats.csv
@@ -0,0 +1,7 @@
+"ID","n_targets","n_bootstraps","n_processed","n_pseudoaligned","n_unique","p_pseudoaligned","p_unique","kallisto_version","index_version","start_time","call"
+"DB_097",31434,100,11071656,7663654,7522185,69.2,67.9,"0.44.0",10,"Fri Aug  6 15:48:13 2021","kallisto quant --single -b 100 -t 30 -l 200 -s 20 -i /Users/dominikbrilhaus/sciebo/CEPLAS_DM/CEPLAS_ARCs/ARC0005//runs/01_kallisto_index -o /Users/dominikbrilhaus/sciebo/CEPLAS_DM/CEPLAS_ARCs/ARC0005//runs/01_kallisto_results/DB_097 /Users/dominikbrilhaus/sciebo/CEPLAS_DM/CEPLAS_ARCs/ARC0005/assays/Talinum_RNASeq_minimal/dataset/DB_097_CAMMD_CAGATC_L001_R1_001.fastq.gz"
+"DB_099",31434,100,14332278,10066506,9848811,70.2,68.7,"0.44.0",10,"Fri Aug  6 15:49:19 2021","kallisto quant --single -b 100 -t 30 -l 200 -s 20 -i /Users/dominikbrilhaus/sciebo/CEPLAS_DM/CEPLAS_ARCs/ARC0005//runs/01_kallisto_index -o /Users/dominikbrilhaus/sciebo/CEPLAS_DM/CEPLAS_ARCs/ARC0005//runs/01_kallisto_results/DB_099 /Users/dominikbrilhaus/sciebo/CEPLAS_DM/CEPLAS_ARCs/ARC0005/assays/Talinum_RNASeq_minimal/dataset/DB_099_CAMMD_CTTGTA_L001_R1_001.fastq.gz"
+"DB_103",31434,100,13083957,9007720,8828236,68.8,67.5,"0.44.0",10,"Fri Aug  6 15:50:41 2021","kallisto quant --single -b 100 -t 30 -l 200 -s 20 -i /Users/dominikbrilhaus/sciebo/CEPLAS_DM/CEPLAS_ARCs/ARC0005//runs/01_kallisto_index -o /Users/dominikbrilhaus/sciebo/CEPLAS_DM/CEPLAS_ARCs/ARC0005//runs/01_kallisto_results/DB_103 /Users/dominikbrilhaus/sciebo/CEPLAS_DM/CEPLAS_ARCs/ARC0005/assays/Talinum_RNASeq_minimal/dataset/DB_103_CAMMD_AGTCAA_L001_R1_001.fastq.gz"
+"DB_161",31434,100,12785081,9058396,8740183,70.9,68.4,"0.44.0",10,"Fri Aug  6 15:51:58 2021","kallisto quant --single -b 100 -t 30 -l 200 -s 20 -i /Users/dominikbrilhaus/sciebo/CEPLAS_DM/CEPLAS_ARCs/ARC0005//runs/01_kallisto_index -o /Users/dominikbrilhaus/sciebo/CEPLAS_DM/CEPLAS_ARCs/ARC0005//runs/01_kallisto_results/DB_161 /Users/dominikbrilhaus/sciebo/CEPLAS_DM/CEPLAS_ARCs/ARC0005/assays/Talinum_RNASeq_minimal/dataset/DB_161_reC3MD_GTCCGC_L001_R1_001.fastq.gz"
+"DB_163",31434,100,14312514,10054493,9707941,70.2,67.8,"0.44.0",10,"Fri Aug  6 15:53:12 2021","kallisto quant --single -b 100 -t 30 -l 200 -s 20 -i /Users/dominikbrilhaus/sciebo/CEPLAS_DM/CEPLAS_ARCs/ARC0005//runs/01_kallisto_index -o /Users/dominikbrilhaus/sciebo/CEPLAS_DM/CEPLAS_ARCs/ARC0005//runs/01_kallisto_results/DB_163 /Users/dominikbrilhaus/sciebo/CEPLAS_DM/CEPLAS_ARCs/ARC0005/assays/Talinum_RNASeq_minimal/dataset/DB_163_reC3MD_GTGAAA_L001_R1_001.fastq.gz"
+"DB_165",31434,100,13679479,9552504,9264346,69.8,67.7,"0.44.0",10,"Fri Aug  6 15:54:35 2021","kallisto quant --single -b 100 -t 30 -l 200 -s 20 -i /Users/dominikbrilhaus/sciebo/CEPLAS_DM/CEPLAS_ARCs/ARC0005//runs/01_kallisto_index -o /Users/dominikbrilhaus/sciebo/CEPLAS_DM/CEPLAS_ARCs/ARC0005//runs/01_kallisto_results/DB_165 /Users/dominikbrilhaus/sciebo/CEPLAS_DM/CEPLAS_ARCs/ARC0005/assays/Talinum_RNASeq_minimal/dataset/DB_165_re-C3MD_GTGAAA_L002_R1_001.fastq.gz"
diff --git a/runs/kallisto_sleuth/run2/03_kallisto_tpmMatrix.csv b/runs/kallisto_sleuth/run2/03_kallisto_tpmMatrix.csv
new file mode 100644
index 0000000000000000000000000000000000000000..b36c4780c19ab8ac4aedb712754668b9c6451371
--- /dev/null
+++ b/runs/kallisto_sleuth/run2/03_kallisto_tpmMatrix.csv
@@ -0,0 +1,31435 @@
+"target_id","CAM_01","CAM_02","CAM_03","reC3_01","reC3_02","reC3_03"
+"Tfru00001_t",2.38259718580395,2.52445339258139,2.99559686081831,4.485419618992,5.15554766862727,4.81121852299496
+"Tfru00002_t",25.1245986606198,25.2087869222372,26.5380313245026,30.658236957757,29.3913436146477,20.8234431654784
+"Tfru00003_t",0,0,0,0.309365430148544,0.725542630523583,1.05024822889448
+"Tfru00004_t",0.103844717888069,0.0833541471161738,0.0894261013194486,0.107415134520747,0.449405712721379,0.0759765881661035
+"Tfru00005_t",0.103461055137251,0.996554261334748,0.623669965236499,113.224757627604,148.648845382204,129.135350356906
+"Tfru00006_t",5.25064854821547,8.28385729734509,7.17220460021974,9.17681755934687,14.8875327806903,12.3195055255455
+"Tfru00007_t",3.06013893760537,4.10665056164903,3.74698927321371,6.23178959717579,6.24916931819361,7.38140445268395
+"Tfru00008_t",14.2043439781122,26.3981885595812,13.4442914669108,26.9366560835528,22.7611500493748,19.0527641655261
+"Tfru00009_t",6.98648923869327,9.55423377037359,10.3244912930937,13.6504343999739,14.7070095797832,16.21819340388
+"Tfru00010_t",244.725880160303,254.236879163414,239.055409283695,234.24905727355,255.175913290995,153.47176428698
+"Tfru00011_t",249.648439921802,235.855086169051,261.859652611709,239.044084421155,277.23708760388,237.068812271962
+"Tfru00012_t",0,0,0,0,0,0
+"Tfru00013_t",0,0,0,0,0,0
+"Tfru00014_t",0.0481534782380506,0.0385649628570616,0.040081234898993,0,0.0409396548817672,0.0683249248759905
+"Tfru00015_t",9.12352121874593,7.38645814357469,6.05895371227259,8.36401371837615,8.18030748581089,6.6074939313356
+"Tfru00016_t",0,0,0,0,0,0
+"Tfru00017_t",73.8872427283506,59.7748520926179,84.9711456238005,79.6773332599202,64.3547735725843,54.9950535288643
+"Tfru00018_t",1.17497030449515,1.88625188858599,2.02365639069639,1.82305266770431,1.01697684361232,3.00877915233859
+"Tfru00019_t",100.042891419037,88.5386478055069,90.5702092996186,107.46269236286,116.564177910843,73.6642320031186
+"Tfru00020_t",4.02402645039706,3.40000865631855,5.41073001773245,4.81959974168133,4.94942973931415,4.2870587946129
+"Tfru00021_t",83.6566488692519,95.2586986660982,83.0071898994768,27.350267688798,24.8757888457407,26.3329256308797
+"Tfru00022_t",0,0,0,0,0,0
+"Tfru00023_t",0,0,0,0.0722351154009016,0,0
+"Tfru00024_t",0,0,0,0,0,0
+"Tfru00025_t",10.3672379845616,10.3302390664317,10.2823272906906,11.7590775902453,8.66377719537418,6.800389597812
+"Tfru00026_t",1.8199540035594,2.42604099287411,1.93108487495278,3.59697341954331,3.99433883682836,2.59175687469059
+"Tfru00027_t",55.063462876232,42.4428339387146,49.6338143237525,83.0396302423057,82.6243070202033,67.3009460579787
+"Tfru00028_t",21.6026683126579,10.7893608027175,14.2624411478655,16.3866391217165,13.9195073409354,13.6979276184499
+"Tfru00029_t",3.29320676944074,2.53549978863862,2.9517052114713,38.5135675842526,37.1713172155247,30.1424653388556
+"Tfru00030_t",21.6360831128322,23.8598828656384,22.7863826973994,22.4807913845528,21.2574115640561,19.0384814079383
+"Tfru00031_t",86.4958908771116,87.5837535575628,99.1007884405973,14.1403444097653,15.9195990519397,11.2746559444835
+"Tfru00032_t",21.9121553586945,23.561903819911,19.2257316055188,25.4025163550232,23.2598419325332,12.7043922869481
+"Tfru00033_t",4.71864451029055,3.49050081035052,2.6293046552041,2.20118216743122,1.76178917951874,1.15077755181544
+"Tfru00034_t",0.172293635708465,0.110637415942551,0.0890226201404027,0.926730902177769,1.37195932676074,1.18492937319104
+"Tfru00035_t",16.8182467453908,22.2939451138924,11.523788250828,15.2219341449141,43.285382782368,59.3057964157302
+"Tfru00036_t",3.22627015393128,3.13485723171429,2.70519604931284,2.54680795451365,2.05759236070029,3.23009915206836
+"Tfru00037_t",31.8300185283124,29.8215252000578,28.399063659266,19.4847718146893,21.5883339032028,21.3409741881647
+"Tfru00038_t",3.0807064823057,3.53260503581409,3.41094469927948,8.80116346097062,7.83007054650118,9.69559441964398
+"Tfru00039_t",244.104882716102,240.53698169767,241.602928149067,96.477604753599,106.200930024934,110.901226580709
+"Tfru00040_t",17.8020185223536,16.7823691475994,17.1568289444861,23.8991409984868,23.0140300773039,22.0586959521175
+"Tfru00041_t",0,0,0,0.414791617314008,0.347082789108922,0.146694653523574
+"Tfru00042_t",6.27821591939822,5.35436518117112,5.57545218805539,6.12878347404016,5.73967290508917,7.34939528984245
+"Tfru00043_t",13.5654149460977,7.45016061094164,12.2967222607536,5.16961960445603,2.88383518542908,1.74121982510985
+"Tfru00044_t",6.97559085105881,7.30326769137017,7.44351229267822,2.19599966350539,1.18127183910627,1.10947761419454
+"Tfru00045_t",12.0424430866921,12.0252581472268,14.2592665430693,23.8007925499645,20.2258645458806,19.8764997220112
+"Tfru00046_t",4.5875973663589,4.83311810701937,5.67901524659038,8.00774328783852,4.96340234254738,4.82490230719006
+"Tfru00047_t",5.27537687732794,5.66356753282366,5.22433741680056,9.41290459039661,7.30562957044336,5.9824705011545
+"Tfru00048_t",12.7695772661346,17.2629772801229,21.993097648717,35.4547282728544,47.1185711067383,42.2879611501977
+"Tfru00049_t",13.5348030138495,11.7694683940428,12.3448298359328,12.7205756663587,10.3292078546594,12.2184765960621
+"Tfru00050_t",4.4215987774446,3.43822147290546,3.3317106037634,40.0192043941442,24.0386228749706,12.8389187466282
+"Tfru00051_t",20.3170495752907,21.1104889593231,18.462111493173,23.4652664340739,26.8631264618122,19.4244180163478
+"Tfru00052_t",29.9398683174839,16.8583762542462,31.5550463262212,16.6402041371396,13.5371518677722,14.058497726246
+"Tfru00053_t",6.07474136968426,6.42583848256019,6.93448286459148,5.30940245357288,4.68727135460016,3.61761843148524
+"Tfru00054_t",0,0.242566564809297,0.520472776744129,0.937757307488683,0,0
+"Tfru00055_t",7.79317038696173,7.38140407421538,7.11377169995176,7.09378316957426,8.49902076447885,9.35089963963446
+"Tfru00056_t",3.74060801683087,2.75640604448988,3.11561842487489,3.04463102460559,4.45836709688963,6.77461608305331
+"Tfru00057_t",0,0,0,0,0,0
+"Tfru00058_t",0.0876020612840953,0.12305384127187,0.0565790270861057,0.52103060688935,0.265378993862478,0.096139301084011
+"Tfru00059_t",0.311534153664206,1.00024976539409,0.268278303958346,32.0097100871827,20.1333759299178,18.7662172770276
+"Tfru00060_t",0,0,0,0,0,0
+"Tfru00061_t",0,0,0,4.5207491008414,2.52186743300529,3.90818299474078
+"Tfru00062_t",0,0,0,0,0,0
+"Tfru00063_t",2.38768446042566,2.70571503292415,2.83369892970126,13.4488776689946,11.0277743353553,8.96946538187647
+"Tfru00064_t",46.7357591265854,40.7162954308794,45.0565585197451,38.0845679188778,29.4012559116134,27.7718655660567
+"Tfru00065_t",17.3575158618693,14.9774830357972,15.620097765696,11.4009846756871,13.7465222213351,14.6043014050278
+"Tfru00066_t",7.41468102679172,6.49764137410621,8.37687237517656,7.03634362973965,7.30082323519965,8.11239251149762
+"Tfru00067_t",14.4623420887289,12.4701371462261,13.4478462769232,11.4347887757398,8.47670174902154,10.4633908934715
+"Tfru00068_t",24.2691501744134,28.4597144250997,23.7438642980361,23.9985290384902,23.3714300467181,21.9288033286706
+"Tfru00069_t",11.0000750073012,10.9936522941682,11.701617730291,14.72483618126,17.921769074605,21.698191851417
+"Tfru00070_t",0,0,0.116686638657151,0,0,0.0991371932683511
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diff --git a/runs/kallisto_sleuth/run2/04_sleuth_dge.csv b/runs/kallisto_sleuth/run2/04_sleuth_dge.csv
new file mode 100644
index 0000000000000000000000000000000000000000..56afd6f749d0e0f81c2eeedaf0667728152f8eec
--- /dev/null
+++ b/runs/kallisto_sleuth/run2/04_sleuth_dge.csv
@@ -0,0 +1,19714 @@
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diff --git a/runs/kallisto_sleuth/run2/05_shinyPrep.RData b/runs/kallisto_sleuth/run2/05_shinyPrep.RData
new file mode 100644
index 0000000000000000000000000000000000000000..e05d66658d5b95026e2731cf8c6ac993fe04f4c2
Binary files /dev/null and b/runs/kallisto_sleuth/run2/05_shinyPrep.RData differ
diff --git a/runs/run1/01_kallisto_index b/runs/run1/01_kallisto_index
deleted file mode 100644
index f26971208cd28e359aa31068043174b317097675..0000000000000000000000000000000000000000
--- a/runs/run1/01_kallisto_index
+++ /dev/null
@@ -1,3 +0,0 @@
-version https://git-lfs.github.com/spec/v1
-oid sha256:179d159502ea85576e5028a32e698c44c1bb076602e17de09f62b9a9b937f8d7
-size 750438441
diff --git a/workflows/.Rhistory b/workflows/.Rhistory
new file mode 100644
index 0000000000000000000000000000000000000000..b03a7c2244a8e54147181a766acf9ee94e879926
--- /dev/null
+++ b/workflows/.Rhistory
@@ -0,0 +1,131 @@
+# ### sleuth installation
+#
+if (!requireNamespace("BiocManager", quietly = TRUE))
+install.packages("BiocManager")
+if (!requireNamespace("BiocManager", quietly = TRUE)) install.packages("BiocManager")
+BiocManager::install()
+BiocManager::install("devtools")    # only if devtools not yet installed
+BiocManager::install("pachterlab/sleuth")
+library(sleuth)
+BiocManager::install("pachterlab/sleuth")
+library(sleuth)
+BiocManager::install("pachterlab/sleuth")
+install.packages('Matrix', dependencies = TRUE)
+BiocManager::install("pachterlab/sleuth")
+source("http://bioconductor.org/biocLite.R")
+devtools::install_github("pachterlab/sleuth")
+remotes::install_github("pachterlab/sleuth#260")
+library(sleuth)
+# BiocManager::install("pachterlab/sleuth")
+# There's currently (early 2022) an issue with sleuth installation.
+# Using this workaround from https://github.com/pachterlab/sleuth/issues/259#issuecomment-1001076030
+remotes::install_github("pachterlab/sleuth#260")
+library(sleuth)
+library(tidyverse)
+library(jsonlite)
+library(openxlsx)
+?commandArgs
+BiocManager::install()
+library(sleuth)
+required_packages <-
+c('tidyverse',  ## data wrangling and plotting
+'jsonlite',   ## read/write json files
+'openxlsx')   ## read/write xlsx files
+for(package in required_packages)
+{
+print(package)
+## Check if package is installed. If not, install
+if(!package %in% row.names(installed.packages())){install.packages(package,
+repos ="https://cran.uni-muenster.de/")}
+# ## Check if package is up to date. If not, update
+# update.packages(package, repos = "https://cran.uni-muenster.de/")
+## Load package
+library(package, character.only = T)
+}
+for(package in required_packages)
+{
+## Check if package is installed. If not, install
+if(!package %in% row.names(installed.packages())){install.packages(package,
+repos ="https://cran.uni-muenster.de/")}
+# ## Check if package is up to date. If not, update
+# update.packages(package, repos = "https://cran.uni-muenster.de/")
+## Load package
+library(package, character.only = T)
+print(paste(package, "installed"))
+}
+{
+## Check if package is installed. If not, install
+if(!package %in% row.names(installed.packages())){install.packages(package,
+repos ="https://cran.uni-muenster.de/")}
+# ## Check if package is up to date. If not, update
+# update.packages(package, repos = "https://cran.uni-muenster.de/")
+## Load package
+library(package, character.only = T)
+print(paste("installed R package", package))
+}
+for(package in required_packages)
+{
+## Check if package is installed. If not, install
+if(!package %in% row.names(installed.packages())){install.packages(package,
+repos ="https://cran.uni-muenster.de/")}
+# ## Check if package is up to date. If not, update
+# update.packages(package, repos = "https://cran.uni-muenster.de/")
+## Load package
+library(package, character.only = T)
+print(paste("installed R package", package))
+}
+run_name="run1"
+arc_root="~/03DataPLANT_gitlab/samplearc_rnaseq/"
+# Load the sleuth object
+load(file = paste0(arc_root, 'runs/', run_name, '/03_kallisto_sleuthObject.RData'))
+so <- sleuth_fit(so)
+so <- sleuth_fit(so, ~Group, 'full')
+so <- sleuth_fit(so, ~Photosynthesis.mode, 'full')
+so <- sleuth_fit(so, ~1, 'reduced')
+so <- sleuth_lrt(so, 'reduced', 'full')
+sleuth_table <- sleuth_results(so, 'reduced:full', 'lrt', show_all = FALSE)
+write.csv(sleuth_table, paste0(arc_root, 'runs/', run_name, '/04_sleuth_dge.csv'), row.names = F)
+library(openxlsx)
+expression_data <- read.csv(file = paste0(arc_root, 'runs/', run_name, '/03_kallisto_df.csv'))
+available_genes <- unique(expression_data$target_id)
+save(expression_data, available_genes, file = paste0(arc_root, 'runs/', run_name, '/05_shinyPrep.RData'))
+load(file = "../runs/run1/05_shinyPrep.RData")
+required.packages <- c('knitr', 'kableExtra', ## RMarkdown,
+"shiny", "tidyverse", ## data loading and shaping
+"RColorBrewer", "shiny" ## plotting
+)
+for(package in required.packages)
+{
+print(package)
+## Check if package is installed. If not, install
+if(!package %in% row.names(installed.packages()))
+{install.packages(package, repos ="https://cran.uni-muenster.de/")}
+## Load package
+library(package, character.only = T)
+}
+required.packages <- c('kableExtra', ## RMarkdown,
+"shiny", "tidyverse", ## data loading and shaping
+"RColorBrewer", "shiny" ## plotting
+)
+for(package in required.packages)
+{
+print(package)
+## Check if package is installed. If not, install
+if(!package %in% row.names(installed.packages()))
+{install.packages(package, repos ="https://cran.uni-muenster.de/")}
+## Load package
+library(package, character.only = T)
+}
+current_selection <- sample(expression_data$target_id, 10)
+plot_set <- subset(expression_data, target_id %in% current_selection)
+ggplot(plot_set, aes(x = Group, y = tpm, group = Group)) +
+stat_summary(fun = 'mean', geom = 'bar') +
+geom_point(size = 0.5) +
+facet_wrap(~ target_id , scales = "free") +
+theme_minimal()
+plot_set <- subset(expression_data, target_id %in% current_selection)
+ggplot(plot_set, aes(x = Photosynthesis.mode, y = tpm, group = Photosynthesis.mode)) +
+stat_summary(fun = 'mean', geom = 'bar') +
+geom_point(size = 0.5) +
+facet_wrap(~ target_id , scales = "free") +
+theme_minimal()
diff --git a/workflows/kallisto_sleuth/01_KallistoIndex.sh b/workflows/kallisto_sleuth/01_KallistoIndex.sh
new file mode 100755
index 0000000000000000000000000000000000000000..ec37c24ab97dbfffeb68fa38476cd435af4fd046
--- /dev/null
+++ b/workflows/kallisto_sleuth/01_KallistoIndex.sh
@@ -0,0 +1,25 @@
+
+run_name=$1
+arc_root=$2
+
+## Manual: http://pachterlab.github.io/kallisto/manual.html
+
+## MacOS Installation 
+# ruby -e "$(curl -fsSL https://raw.githubusercontent.com/Homebrew/install/master/install)"
+# brew install kallisto
+
+
+# git clone https://github.com/pachterlab/kallisto.git
+# cd kallisto
+# mkdir build
+# cd build
+# cmake  .. -DUSE_HDF5=ON
+
+
+kallisto version
+kallisto cite
+
+### Build index 
+
+kall_ref=$arc_root'/studies/TalinumGenomeDraft/resources/Talinum.gm.CDS.nt.fa'
+kallisto index -i $arc_root'/runs/'$run_name'/01_kallisto_index' $kall_ref
\ No newline at end of file
diff --git a/workflows/kallisto_sleuth/01_KallistoQuant.sh b/workflows/kallisto_sleuth/01_KallistoQuant.sh
old mode 100644
new mode 100755
index bca3fd80d639a2bdb2232e5d6c8c59f5319f4eb6..f65e0ac4c0df747d4af8fc4630295cfc54119fda
--- a/workflows/kallisto_sleuth/01_KallistoQuant.sh
+++ b/workflows/kallisto_sleuth/01_KallistoQuant.sh
@@ -1,32 +1,26 @@
-#!/usr/bin/bash
+#!/usr/bin/env bash
+
 # Map RNASeq reads via kallisto
 
 run_name=$1
 arc_root=$2
 
-## Manual: http://pachterlab.github.io/kallisto/manual.html
-
-kallisto version
-kallisto cite
-
-### Build index 
-
-kall_ref=$arc_root'/studies/TalinumGenomeDraft/resources/Talinum.gm.CDS.nt.fa'
-kallisto index -i $arc_root'/runs/'$run_name'/01_kallisto_index' $kall_ref
-
 ### Align reads
 
-ILLUMINASAMPLES=$(ls ${arc_root}'/assays/Talinum_RNASeq_minimal/dataset/'*fastq.gz)
+ILLUMINASAMPLES=$(ls ${arc_root}"/assays/Talinum_RNASeq_minimal/dataset/"*"fastq.gz")
 
-mkdir $arc_root'/runs/'$run_name'/01_kallisto_results/'
+mkdir -p $arc_root'/runs/'$run_name'/01_kallisto_results/'
 
 for j in $ILLUMINASAMPLES; do
 
 	sampleName=$(echo $j | sed -e 's|.*/||' | cut -c -6)  # cut away path. retain only first six chars of file name
 	echo $sampleName
 	
-	kallisto quant --single -b 100 -t 30 -l 200 -s 20 -i $arc_root'/runs/'$run_name'/01_kallisto_index' -o $arc_root'/runs/'$run_name'/01_kallisto_results/'$sampleName $j
+	kallisto quant --single -b 100 -t 8 -l 200 -s 20 -i $arc_root'/runs/'$run_name'/01_kallisto_index' -o $arc_root'/runs/'$run_name'/01_kallisto_results/'$sampleName $j
 
 	echo 'Kallisto done'
 
-done
\ No newline at end of file
+done
+
+
+
diff --git a/workflows/kallisto_sleuth/02_InstallPackages.R b/workflows/kallisto_sleuth/02_InstallPackages.R
index f328f617d63b25b4f5b537ad96aca3fda6c90c50..163e75e2125be651e24cee07081b7eda37a0c477 100644
--- a/workflows/kallisto_sleuth/02_InstallPackages.R
+++ b/workflows/kallisto_sleuth/02_InstallPackages.R
@@ -12,13 +12,15 @@
 
 ### sleuth installation
 
-if (!requireNamespace("BiocManager", quietly = TRUE)) install.packages("BiocManager")
-BiocManager::install()
-BiocManager::install("devtools")    # only if devtools not yet installed
+if (!requireNamespace("BiocManager", quietly = TRUE)){install.packages("BiocManager")}
+if(!"BiocManager" %in% row.names(installed.packages())){BiocManager::install()}
+if(!"devtools" %in% row.names(installed.packages())){BiocManager::install("devtools")}
+
 # BiocManager::install("pachterlab/sleuth")
 # There's currently (early 2022) an issue with sleuth installation. 
 # Using this workaround from https://github.com/pachterlab/sleuth/issues/259#issuecomment-1001076030
-remotes::install_github("pachterlab/sleuth#260")
+
+if(!"sleuth" %in% row.names(installed.packages())){remotes::install_github("pachterlab/sleuth#260")}
 
 library(sleuth)
 
diff --git a/workflows/kallisto_sleuth/03_KallistoCollect.R b/workflows/kallisto_sleuth/03_KallistoCollect.R
index ff069265dcd52435dceaccd2253d5bacc16562ea..f36e8f7d960afa0d05dc5bdd2aa2bd43ec01bc5c 100644
--- a/workflows/kallisto_sleuth/03_KallistoCollect.R
+++ b/workflows/kallisto_sleuth/03_KallistoCollect.R
@@ -45,7 +45,12 @@ assay_data <- merge(study_data, assay_data,  by.x = "Sample.Name", by.y = "Sourc
 assay_data <- assay_data[, !apply(assay_data, 2, function(x){sum(is.na(x)) == nrow(assay_data)})]  
 
 # Pointer to kallisto results folder
-base_dir <- paste0(arc_root, 'runs/', run_name, '/01_kallisto_results/')
+# base_dir <- paste0(arc_root, 'runs/', run_name, '/01_kallisto_results/')
+
+########################
+### Note: hard coded link due to issues with kallisto version
+base_dir <- paste0(arc_root, 'runs/kallisto_sleuth/run1/01_kallisto_results')
+########################
 
 # A list of paths to the kallisto results indexed by the sample IDs is collated with
 kal_dirs <- dir(base_dir, full.names = T) ## Sleuth requires full paths
@@ -58,7 +63,7 @@ s2c$path <- kal_dirs
 s2c <- s2c[order(s2c$sample), ]
 
 # Build a sleuth object
-so <- sleuth_prep(s2c, ~Photosynthesis.mode)
+so <- sleuth_prep(s2c, ~Photosynthesis.mode, num_cores = 1)
 save(so, file = paste0(arc_root, 'runs/', run_name, '/03_kallisto_sleuthObject.RData'))
 
 # Extract expression tables
diff --git a/workflows/kallisto_sleuth_wrapper.sh b/workflows/kallisto_sleuth_wrapper.sh
index d0bbe3114bec8a6a30b98627e78b78ae3f451f5d..641796bbe02cfbd66ab214fc6718ec3e4ff95733 100755
--- a/workflows/kallisto_sleuth_wrapper.sh
+++ b/workflows/kallisto_sleuth_wrapper.sh
@@ -1,4 +1,4 @@
- #!/usr/bin/bash
+#!/usr/bin/env bash
 
 #######################################
 #### To be replaced by CWL routine #### 
@@ -8,21 +8,30 @@
 # Execute within <ARC root>/workflows: 
 ## change rights: `chmod a+x kallisto_sleuth_wrapper.sh`
 ## execute script: `./kallisto_sleuth_wrapper.sh <run_name> <arc_root>`
-## Example: ./kallisto_sleuth_wrapper.sh "run2" "~/03DataPLANT_gitlab/samplearc_rnaseq/"
+## Example: ./kallisto_sleuth_wrapper.sh "run2" "/Users/dominikbrilhaus/03DataPLANT_gitlab/samplearc_rnaseq/"
 
 run_name='kallisto_sleuth/'$1
 arc_root=$2
 
-mkdir $arc_root'/workflows/'$run_name
+# Note: due to some not well documented change in kallisto development (use of hd5) the kallisto quant part is currently not reproducible and commented out 
+# Pointer to `runs/kallisto_sleuth/run1/01_kallisto_results` is thus hard coded into 03_KallistoCollect.R
 
-# chmod a+x 01_KallistoQuant.sh 
-# ./01_KallistoQuant.sh $run_name $arc_root
+mkdir -p $arc_root'/runs/'$run_name
 
-# Rscript 02_InstallPackages.R
-# Rscript 03_KallistoCollect.R $run_name $arc_root
-# Rscript 04_Sleuth.R $run_name $arc_root
-# Rscript 05_plot_shinyPrep.R $run_name $arc_root
+# chmod a+x ./kallisto_sleuth/01_KallistoIndex.sh
+# ./kallisto_sleuth/01_KallistoIndex.sh $run_name $arc_root
+# chmod a+x kallisto_sleuth/01_KallistoQuant.sh 
+# ./kallisto_sleuth/01_KallistoQuant.sh $run_name $arc_root
+
+Rscript ./kallisto_sleuth/02_InstallPackages.R
+echo "r insallations done"
+Rscript ./kallisto_sleuth/03_KallistoCollect.R $run_name $arc_root
+echo "kallisto collect done"
+Rscript ./kallisto_sleuth/04_Sleuth.R $run_name $arc_root
+echo "sleuth done"
+Rscript ./kallisto_sleuth/05_plot_shinyPrep.R $run_name $arc_root
+echo "shiny prep done"
 
 # store variables for test runs in R
-# run_name="run1"
-# arc_root="~/03DataPLANT_gitlab/samplearc_rnaseq/"
\ No newline at end of file
+# run_name="kallisto_sleuth/run2"
+# arc_root="/Users/dominikbrilhaus/03DataPLANT_gitlab/samplearc_rnaseq/"
\ No newline at end of file
diff --git a/workflows/plot_shinyApp.Rmd b/workflows/plot_shinyApp.Rmd
index 19b291643efdeb4f9973950f9babd178ab501858..a71064203f9bdc293879516523fd44ef5accf128 100644
--- a/workflows/plot_shinyApp.Rmd
+++ b/workflows/plot_shinyApp.Rmd
@@ -14,7 +14,7 @@ knitr::opts_chunk$set(echo = TRUE)
 
 # Load data
 
-load(file = "../runs/run1/05_shinyPrep.RData")
+load(file = "../runs/kallisto_sleuth/run2/05_shinyPrep.RData")
 
 ```