diff --git a/runs/kallisto/README.md b/runs/kallisto/README.md deleted file mode 100644 index e3c73f34783ecbe96fe58e4044df92be08372cf9..0000000000000000000000000000000000000000 --- a/runs/kallisto/README.md +++ /dev/null @@ -1,8 +0,0 @@ -# Kallisto Workflow - -## Executing the multi-step workflow - -```bash -cd runs/kallisto/ -cwltool --cachedir cache2 ../../workflows/kallisto/kallisto-workflow.cwl workflow.yml > $(date +"%Y-%m-%d_%H-%M").log 2>&1 & -``` diff --git a/runs/kallisto/run.cwl b/runs/kallisto/run.cwl new file mode 100644 index 0000000000000000000000000000000000000000..7ab4d83630e9cacda2b7da8c7a9b3ac804c5f12e --- /dev/null +++ b/runs/kallisto/run.cwl @@ -0,0 +1,40 @@ +#!/usr/bin/env cwl-runner +cwlVersion: v1.2 +class: Workflow + +requirements: + SubworkflowFeatureRequirement: {} + +inputs: + IndexInput: File[] + InputReadsMultipleSamples: + type: + type: array + items: + type: array + items: File + numUnderscoresFileName: int + isSingle: boolean + FragmentLength: double? + StandardDeviation: double? + BootstrapSamples: int? + resultsFolder: string + +steps: + kallisto: + run: ../../workflows/kallisto/workflow.cwl + in: + IndexInput: IndexInput + InputReadsMultipleSamples: InputReadsMultipleSamples + numUnderscoresFileName: numUnderscoresFileName + isSingle: isSingle + FragmentLength: FragmentLength + StandardDeviation: StandardDeviation + BootstrapSamples: BootstrapSamples + resultsFolder: resultsFolder + out: [finalOut] + +outputs: + finalOut: + type: Directory[] + outputSource: kallisto/finalOut diff --git a/runs/kallisto/workflow.yml b/runs/kallisto/run.yml similarity index 56% rename from runs/kallisto/workflow.yml rename to runs/kallisto/run.yml index 0f8f15d1452b75328f81888b196b8fd19ac97a11..1d147f9882253552f871d417b493cb2cf9ca5c17 100644 --- a/runs/kallisto/workflow.yml +++ b/runs/kallisto/run.yml @@ -17,22 +17,22 @@ InputReadsMultipleSamples: - class: File path: ../../assays/Talinum_RNASeq_minimal/dataset/DB_099_CAMMD_CTTGTA_L001_R1_001.fastq.gz format: edam:format_1930 # FASTQ - - - - class: File - path: ../../assays/Talinum_RNASeq_minimal/dataset/DB_103_CAMMD_AGTCAA_L001_R1_001.fastq.gz - format: edam:format_1930 # FASTQ - - - - class: File - path: ../../assays/Talinum_RNASeq_minimal/dataset/DB_161_reC3MD_GTCCGC_L001_R1_001.fastq.gz - format: edam:format_1930 # FASTQ - - - - class: File - path: ../../assays/Talinum_RNASeq_minimal/dataset/DB_163_reC3MD_GTGAAA_L001_R1_001.fastq.gz - format: edam:format_1930 # FASTQ - - - - class: File - path: ../../assays/Talinum_RNASeq_minimal/dataset/DB_165_re-C3MD_GTGAAA_L002_R1_001.fastq.gz - format: edam:format_1930 # FASTQ + # - + # - class: File + # path: ../../assays/Talinum_RNASeq_minimal/dataset/DB_103_CAMMD_AGTCAA_L001_R1_001.fastq.gz + # format: edam:format_1930 # FASTQ + # - + # - class: File + # path: ../../assays/Talinum_RNASeq_minimal/dataset/DB_161_reC3MD_GTCCGC_L001_R1_001.fastq.gz + # format: edam:format_1930 # FASTQ + # - + # - class: File + # path: ../../assays/Talinum_RNASeq_minimal/dataset/DB_163_reC3MD_GTGAAA_L001_R1_001.fastq.gz + # format: edam:format_1930 # FASTQ + # - + # - class: File + # path: ../../assays/Talinum_RNASeq_minimal/dataset/DB_165_re-C3MD_GTGAAA_L002_R1_001.fastq.gz + # format: edam:format_1930 # FASTQ numUnderscoresFileName: 2 @@ -42,7 +42,7 @@ FragmentLength: 200 StandardDeviation: 20 BootstrapSamples: 30 -resultsFolder: kallisto_results +resultsFolder: test $namespaces: edam: https://edamontology.org/ \ No newline at end of file diff --git a/workflows/.gitkeep b/workflows/.gitkeep deleted file mode 100644 index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..0000000000000000000000000000000000000000 diff --git a/workflows/kallisto/kallisto-index.cwl b/workflows/kallisto/kallisto-index.cwl index 3d29b9edba1ec8b2f5e1033659ecd36f394dba56..d35f52022fabbb4028a84fe2866fbf3343122947 100644 --- a/workflows/kallisto/kallisto-index.cwl +++ b/workflows/kallisto/kallisto-index.cwl @@ -4,7 +4,7 @@ class: CommandLineTool hints: DockerRequirement: - dockerPull: quay.io/biocontainers/kallisto:0.50.1--h6de1650_2 + dockerPull: quay.io/biocontainers/kallisto:0.51.1--ha4fb952_1 SoftwareRequirement: packages: kallisto: diff --git a/workflows/kallisto/kallisto-quant.cwl b/workflows/kallisto/kallisto-quant.cwl index 6d3e3578d1e8a43199e2aa18afe8d22888c2f551..026f7e1bc37dba078b750b3f7ecb70019c4bc753 100755 --- a/workflows/kallisto/kallisto-quant.cwl +++ b/workflows/kallisto/kallisto-quant.cwl @@ -4,7 +4,7 @@ class: CommandLineTool hints: DockerRequirement: - dockerPull: quay.io/biocontainers/kallisto:0.50.1--h6de1650_2 + dockerPull: quay.io/biocontainers/kallisto:0.51.1--ha4fb952_1 SoftwareRequirement: packages: kallisto: diff --git a/workflows/kallisto/kallisto-workflow.cwl b/workflows/kallisto/workflow.cwl similarity index 100% rename from workflows/kallisto/kallisto-workflow.cwl rename to workflows/kallisto/workflow.cwl