diff --git a/.gitattributes b/.gitattributes index 2635f9cbbd98377405c62ee003f1d6b4b772e7b2..60db50c94ee4d373a2a2e34c05d5aed40d8a7707 100644 --- a/.gitattributes +++ b/.gitattributes @@ -1 +1,2 @@ **/dataset/** filter=lfs diff=lfs merge=lfs -text +/runs/run1/01_kallisto_index filter=lfs diff=lfs merge=lfs -text diff --git a/README.md b/README.md index 49cbdc8bc92f29f2d766dc4926fe13814fd96e23..eba9e1a0daa42bde35cf79cd4c18ecdb31cb1b30 100644 --- a/README.md +++ b/README.md @@ -1,12 +1,14 @@ # ARC mininmal Example RNASeq -This is a minimal Example ARC packaging an mRNA-Seq dataset with metadata and computations. +This is a minimal Example ARC packaging an mRNA-Seq dataset with metadata and computations. ## Data origin -- Partly published under: https://doi.org/10.1104/pp.15.01076 -- see [./isa.investigation.xlsx](./isa.investigation.xlsx) for details. + +- Partly published under: <https://doi.org/10.1104/pp.15.01076> +- see [./isa.investigation.xlsx](./isa.investigation.xlsx) for details. ## Additional payload + > The following folders are not part of the ARC > for details, see: [ARC specs:Additional paylod](https://github.com/nfdi4plants/ARC-specification/blob/main/ARC%20specification.md#additional-payload) @@ -14,34 +16,33 @@ Directory |Â Purpose ---- | ---- [_GEO_submission](./_GEO_submission) | Example metadata files as required for submission to GEO - ## Notes and ToDos ### Experimental metadata in isa.assay.xlsx -- split GEO SWATE templates into four sheets - - 1SPL01_plants - - 2EXT01_RNA - - 3ASY01_RNASeq - - 4COM01_RNASeq -### Adding large raw data via git lfs +- split GEO SWATE templates into four sheets + - 1SPL01_plants + - 2EXT01_RNA + - 3ASY01_RNASeq + - 4COM01_RNASeq -1. Before addind the files to the ARC, track them via `git lfs` - -``` -git lfs track "*.fastq.gz" -git lfs track "01_kallisto_index" +### Adding large raw data via git lfs -``` +1. Before addind the files to the ARC, track them via `git lfs` -2. Move / add the large data files to the respective folders + ```bash + git lfs track "*.fastq.gz" + git lfs track "01_kallisto_index" + ``` -3. Add them via `git add` +1. Move / add the large data files to the respective folders -``` -git add assays/Talinum_RNASeq_minimal/dataset/ -git add runs/run1/01_kallisto_index +1. Add them via `git add` -``` + ```bash + git add assays/Talinum_RNASeq_minimal/dataset/ + git add runs/run1/01_kallisto_index + + ``` -4. Commit +1. Commit diff --git a/workflows/.Rhistory b/workflows/.Rhistory new file mode 100644 index 0000000000000000000000000000000000000000..2682a56b8b7906d6b98e05c70905cc6584d6c11f --- /dev/null +++ b/workflows/.Rhistory @@ -0,0 +1 @@ +ARC_root="~/03_DataPLANT_gitlab/samplearc_rnaseq/" diff --git a/workflows/06_plot_shinyApp.Rmd b/workflows/06_plot_shinyApp.Rmd index d48a3b6337495a47fc9b91a8eb759aa5f4fc060e..09b641d24c043e285b0d376c994d81cc54352c7b 100644 --- a/workflows/06_plot_shinyApp.Rmd +++ b/workflows/06_plot_shinyApp.Rmd @@ -14,7 +14,7 @@ knitr::opts_chunk$set(echo = TRUE) # Load data -ARC_root="~/Hackathon_ARCexample_rnaseq/" +ARC_root="~/03_DataPLANT_gitlab/samplearc_rnaseq/" load(file = paste0(ARC_root, 'runs/05_shinyPrep.RData')) ```