diff --git a/studies/TalinumFacultativeCAM/isa.study.xlsx b/studies/TalinumFacultativeCAM/isa.study.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..2b5d5bbefa04909d9f4791ad9db51f14bb830c14 Binary files /dev/null and b/studies/TalinumFacultativeCAM/isa.study.xlsx differ diff --git a/studies/TalinumFacultativeCAM/protocols/plant_material.md b/studies/TalinumFacultativeCAM/protocols/plant_material.md index 0c03bf77b9182231094098c2b5d124f5ba8b0788..6b81fa2e22dd13fdafda15edd57d9c08f4883136 100644 --- a/studies/TalinumFacultativeCAM/protocols/plant_material.md +++ b/studies/TalinumFacultativeCAM/protocols/plant_material.md @@ -1,9 +1,8 @@ -``` -Note: -- This is a somewhat polished protocol as found in a publication. -- This is not an example for a typical lab protocol or method. -``` - # Plant Material and Growth Conditions -*Talinum triangulare* plants were grown in Miracle-Gro Potting Mix (Miracle-Gro) in “Short-One†treepots, 1.6 l (Stuewe and Sons). The experiment was initiated with 28-d-old plants in a controlled environment chamber (Environmental Growth Chambers) maintained under 12 h light (30°C, 37% relative humidity)/12 h dark (22°C) cycles. Photon flux density at leaf level was 425 mmol m<sup>-2</sup> s<sup>-1</sup>. Irrigation was withheld on day 1 and recommenced on day 14. Leaves were harvested when plants were well-watered as well as after 4, 9, and 12 days of water deprivation and watered for two days following the drought period. \ No newline at end of file +*Talinum triangulare* plants were grown in Miracle-Gro Potting Mix (Miracle-Gro) in “Short-One†treepots, 1.6 l (Stuewe and Sons). The experiment was initiated with 28-d-old plants in a controlled environment chamber (Environmental Growth Chambers) maintained under 12 h light (30°C, 37% relative humidity)/12 h dark (22°C) cycles. Photon flux density at leaf level was 425 mmol m<sup>-2</sup> s<sup>-1</sup>. + + +# Treatment and harvest + +Irrigation was withheld on day 1 and recommenced on day 14. Leaves were harvested when plants were well-watered as well as after 4, 9, and 12 days of water deprivation and watered for two days following the drought period. The topmost mature unshaded leaves (of approximately 3–4.5 cm length) of *T. triangulare* were harvested in the middle of the light or the middle of the dark period and immediately frozen in liquid nitrogen.