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diff --git a/assays/Isolation of extracellular polysaccharide matrix/isa.assay.xlsx b/assays/Isolation of extracellular polysaccharide matrix/isa.assay.xlsx
index 9ae2735534d88df36625cefa40e389c7a10f2c8c..245265e1af2ac5d00dac4846571b45a3345c195a 100644
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diff --git a/assays/Isolation of extracellular polysaccharide matrix/protocols/Isolation of extracellular polysaccharide matrix.md b/assays/Isolation of extracellular polysaccharide matrix/protocols/Isolation of extracellular polysaccharide matrix.md
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+## Microbial strains and culture conditions for EPS matrix production in S. indica and B. sorokiniana
+
+The EPS matrix was isolated from the S. indica strain
+expressing GFP in the dikaryotic wild-type background
+DSM11827 (Wawra et al., 2016) and from the B. sorokiniana
+wild-type strain ND90r. Serendipita indica spores were iso-
+lated from 3-week-old cultures grown on solid complex me-
+dium (Si_CM) using 0.002% (v/v) Tween water (Zuccaro
+et al., 2011). For the preculture, 2 mL of S. indica spores at a
+concentration of 500,000 mL–1 were inoculated in 100 mL
+of TSB medium containing 1% (w/v) sucrose and shaking at
+120 rpm at 28	C. After 48 h, the pre-cultures were trans-
+ferred to 400 mL of TSB containing 1% sucrose and shaken
+at 120 rpm at 28	Cfor 72 h. Bipolaris sorokiniana spores
+were isolated from 10-day-old cultures grown on solid com-
+plete medium (Bs_CM), using 0.002% (v/v) Tween water
+(Sarkar et al., 2019). The spores were inoculated at a final
+concentration of 250 spores	mL–1 in 250 mL of YPD me-
+dium and these samples were shaken at 28	C for 36 h.
+
+## Isolation of EPS matrix from S. indica and B. sorokiniana culture supernatant
+
+Culture supernatants from axenically grown S. indica or B.
+sorokiniana were collected by filtering the mycelia using
+Miracloth (Merck Millipore). The EPS matrix was isolated
+from the culture media using cryogelation. Briefly, the cul-
+ture media were frozen overnight at –20°C and slowly
+thawed at room temperature for 16 h. The precipitated EPS matrix (Supplemental Figure S1) present in the culture me-
+dium was isolated using a pipette controller and washed
+four times with 30 mL of Milli-Q water and either used for
+proteome analyses (see section “Proteome analysis of
+S.indica EPS matrix, CW, and culture filtrate”) or washed
+one more time with 30 mL of 8.3-mM EDTA (pH 8.0) to re-
+move metal ions potentially present in the EPS. The proteins
+present in the EPS matrix were removed by treatment with
+30 mL of protein denaturation solution (containing 8-M
+urea, 2-M thiourea, 4% [w/v] sodium dodecyl sulfate [SDS],
+100-mM Tris–HCl, pH 7.5) and boiling at 95°C for 15 min.
+The SDS present in the EPS matrix was removed by boiling
+the material with 30 mL of Milli-Q water at 95°C for 10 min
+and centrifuging at 10,000g for 10 min at room temperature.
+The latter step was repeated approximately 15 times until
+no further foaming occurred. The resulting protein-free EPS
+matrix was lyophilized and used for glycosyl linkage, TLC or
+MALDI-TOF analyses.
\ No newline at end of file
diff --git a/assays/Proteome analysis of S indica EPS matrix CW and cullture filtrate/protocols/Proteome analysis of S. indica EPS matrix, CW, and culture filtrate b/assays/Proteome analysis of S indica EPS matrix CW and cullture filtrate/protocols/Proteome analysis of S. indica EPS matrix, CW, and culture filtrate
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diff --git a/studies/Characterization of fungal components/isa.study.xlsx b/studies/Characterization of fungal components/isa.study.xlsx
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