From e5b1c0e1ccebbbece11d167922f3fa88fa64a467 Mon Sep 17 00:00:00 2001
From: Pia Saake <psaake@uni-koeln.de>
Date: Fri, 11 Oct 2024 11:10:31 +0200
Subject: [PATCH] changed protocol filename

---
 .../{MALDI-TOF analysis of Si EPS and CW.txt => MALDI-TOF.md} | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)
 rename assays/MALDI-TOF analysis of Si EPS and CW/protocols/{MALDI-TOF analysis of Si EPS and CW.txt => MALDI-TOF.md} (91%)

diff --git a/assays/MALDI-TOF analysis of Si EPS and CW/protocols/MALDI-TOF analysis of Si EPS and CW.txt b/assays/MALDI-TOF analysis of Si EPS and CW/protocols/MALDI-TOF.md
similarity index 91%
rename from assays/MALDI-TOF analysis of Si EPS and CW/protocols/MALDI-TOF analysis of Si EPS and CW.txt
rename to assays/MALDI-TOF analysis of Si EPS and CW/protocols/MALDI-TOF.md
index aecab76..11cb825 100644
--- a/assays/MALDI-TOF analysis of Si EPS and CW/protocols/MALDI-TOF analysis of Si EPS and CW.txt	
+++ b/assays/MALDI-TOF analysis of Si EPS and CW/protocols/MALDI-TOF.md	
@@ -1,7 +1,7 @@
-# Analysis of oligosaccharides released from the EPS matrix and CW of S. indica
+## Analysis of oligosaccharides released from the EPS matrix and CW of S. indica
 
 Freeze-dried  EPS  matrix  (1  mg)  or  CW  preparation  (1  mg)isolated fromS. indicawere suspended in 400mLofa2-mM sodium  acetate  buffer,  pH  5.0  (TLE),  2-mM  MES  buffer,  pH5.0  (H.  pomatiab-1,3-glucanase),  or  25-mM  sodium  acetatebuffer,  pH  5.0  (HvBGLUII)  and  incubated  at  70C  overnight.The  suspended  material  was  treated  with  2.5mLofTLE,2.5mLofH.  pomatiab-1,3-glucanase,  or  1mLofHvBGLUII  inthe respective buffers, as described before, in a total reactionvolume  of  50mL.  The  digestion was  performed  at  40Cwithshaking  at  500  rpm  for  16  h.  The  digestion  reaction  wasstopped at 95C for 10 min and centrifuged at 11,000gfor 5min at room temperature.
 
-# Mass spectrometrical analysis
+## Mass spectrometrical analysis
 
 The  oligosaccharides  present  in  the  prepared  samples  wereanalyzed   by   Oligosaccharide   Mass   Profiling   as   described(Gu ̈nl et al., 2011).  Briefly,  the  samples  were  spotted  onto  adried  spot  of  dihydroxy  benzoic  acid  matrix  (10  mgmL–1)and   analyzed   by   MALDI-TOF   mass   spectrometry   (BrukerrapifleX  instrument).  The  machine  was  set  to  linear,  positivereflectron  mode  with  an  accelerating  voltage  of  20,000  V.The spectra from the samples were analyzed using flexanaly-sis software 4.0 (Bruker Daltonics).
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