diff --git a/assays/Isolation of extracellular polysaccharide matrix/README.md b/assays/Isolation of extracellular polysaccharide matrix/README.md new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/Isolation of extracellular polysaccharide matrix/dataset/.gitkeep b/assays/Isolation of extracellular polysaccharide matrix/dataset/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/Isolation of extracellular polysaccharide matrix/isa.assay.xlsx b/assays/Isolation of extracellular polysaccharide matrix/isa.assay.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..9ae2735534d88df36625cefa40e389c7a10f2c8c Binary files /dev/null and b/assays/Isolation of extracellular polysaccharide matrix/isa.assay.xlsx differ diff --git a/assays/Isolation of extracellular polysaccharide matrix/protocols/.gitkeep b/assays/Isolation of extracellular polysaccharide matrix/protocols/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/MALDI-TOF analysis of Si EPS and CW/README.md b/assays/MALDI-TOF analysis of Si EPS and CW/README.md new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/MALDI-TOF analysis of Si EPS and CW/dataset/.gitkeep b/assays/MALDI-TOF analysis of Si EPS and CW/dataset/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/MALDI-TOF analysis of Si EPS and CW/dataset/MALDI_Si_CW.xlsx b/assays/MALDI-TOF analysis of Si EPS and CW/dataset/MALDI_Si_CW.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..c2c89bfe782cc5044d7f357a6ec07f4414ba6fac --- /dev/null +++ b/assays/MALDI-TOF analysis of Si EPS and CW/dataset/MALDI_Si_CW.xlsx @@ -0,0 +1,3 @@ +version https://git-lfs.github.com/spec/v1 +oid sha256:397b5c281c2f57c2ad6e0c572219dca230a8934ffd1c1d5b28711bf82eccce5b +size 16099 diff --git a/assays/MALDI-TOF analysis of Si EPS and CW/dataset/MALDI_Si_EPS.xlsx b/assays/MALDI-TOF analysis of Si EPS and CW/dataset/MALDI_Si_EPS.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..37a220f25352c18459f470800fa76c5ddf796cc7 --- /dev/null +++ b/assays/MALDI-TOF analysis of Si EPS and CW/dataset/MALDI_Si_EPS.xlsx @@ -0,0 +1,3 @@ +version https://git-lfs.github.com/spec/v1 +oid sha256:3bea9ddbbd9fc24e8849e31b081d9055e25e40009dbd7e011d693807928cae07 +size 18410 diff --git a/assays/MALDI-TOF analysis of Si EPS and CW/isa.assay.xlsx b/assays/MALDI-TOF analysis of Si EPS and CW/isa.assay.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..e1ef1190a25f8be7ee8a611c678ede62d7bcd72a Binary files /dev/null and b/assays/MALDI-TOF analysis of Si EPS and CW/isa.assay.xlsx differ diff --git a/assays/MALDI-TOF analysis of Si EPS and CW/protocols/.gitkeep b/assays/MALDI-TOF analysis of Si EPS and CW/protocols/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/MALDI-TOF analysis of Si EPS and CW/protocols/MALDI-TOF analysis of Si EPS and CW.txt b/assays/MALDI-TOF analysis of Si EPS and CW/protocols/MALDI-TOF analysis of Si EPS and CW.txt new file mode 100644 index 0000000000000000000000000000000000000000..aecab76d1f9c6b59d5463ac0ab26ccfb520cc54e --- /dev/null +++ b/assays/MALDI-TOF analysis of Si EPS and CW/protocols/MALDI-TOF analysis of Si EPS and CW.txt @@ -0,0 +1,7 @@ +# Analysis of oligosaccharides released from the EPS matrix and CW of S. indica + +Freeze-dried EPS matrix (1 mg) or CW preparation (1 mg)isolated fromS. indicawere suspended in 400mLofa2-mM sodium acetate buffer, pH 5.0 (TLE), 2-mM MES buffer, pH5.0 (H. pomatiab-1,3-glucanase), or 25-mM sodium acetatebuffer, pH 5.0 (HvBGLUII) and incubated at 70C overnight.The suspended material was treated with 2.5mLofTLE,2.5mLofH. pomatiab-1,3-glucanase, or 1mLofHvBGLUII inthe respective buffers, as described before, in a total reactionvolume of 50mL. The digestion was performed at 40Cwithshaking at 500 rpm for 16 h. The digestion reaction wasstopped at 95C for 10 min and centrifuged at 11,000gfor 5min at room temperature. + +# Mass spectrometrical analysis + +The oligosaccharides present in the prepared samples wereanalyzed by Oligosaccharide Mass Profiling as described(Gu ̈nl et al., 2011). Briefly, the samples were spotted onto adried spot of dihydroxy benzoic acid matrix (10 mgmL–1)and analyzed by MALDI-TOF mass spectrometry (BrukerrapifleX instrument). 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