diff --git a/assays/DeterminationOfBacterialTiter/README.md b/assays/DeterminationOfBacterialTiter/README.md new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/DeterminationOfBacterialTiter/dataset/.gitkeep b/assays/DeterminationOfBacterialTiter/dataset/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/DeterminationOfBacterialTiter/isa.assay.xlsx b/assays/DeterminationOfBacterialTiter/isa.assay.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..8f90326f464ebe2756039f5d7f3f8dbfedd1921e Binary files /dev/null and b/assays/DeterminationOfBacterialTiter/isa.assay.xlsx differ diff --git a/assays/DeterminationOfBacterialTiter/protocols/.gitkeep b/assays/DeterminationOfBacterialTiter/protocols/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/DeterminationOfBacterialTiter/protocols/DeterminationOfBacterialTiterProtocol.md b/assays/DeterminationOfBacterialTiter/protocols/DeterminationOfBacterialTiterProtocol.md new file mode 100644 index 0000000000000000000000000000000000000000..28f372523d58328e39fae1ce5a3c1be1bbbcb8f9 --- /dev/null +++ b/assays/DeterminationOfBacterialTiter/protocols/DeterminationOfBacterialTiterProtocol.md @@ -0,0 +1,3 @@ +## Determination of bacterial titer + +Bacterial titer in the roots was determined using the qPCR method as described in Koprivova et al. (2023). Genomic DNA was extracted by a buffer containing 0.025 M EDTA, 0.2 M Tris pH 8.0, 0.25 M NaCl, and 0.5% SDS. After 10 min incubation at 65 °C and centrifugation, the supernatant was precipitated with isopropanol, washed with 70% ethanol, and resuspended in 100 µl of sterile water. For the qPCR, 13 ng of corresponding DNA samples were used with *Arabidopsis*- (At primer AT4G26410) and *B. glumae* PG1-specific primer (Burk1 for NR042931). The qPCR conditions were the same as for expression analysis. The qPCRs were performed in duplicate for each of the four independent samples. The qPCR results were related to the bacterial titer as established in Koprivova et al. (2023). \ No newline at end of file diff --git a/assays/MetaboliteAnalysis/isa.assay.xlsx b/assays/MetaboliteAnalysis/isa.assay.xlsx index e1b3193b86f7955649c98551e4f877858f149639..c0c917033f350ab4d9b0f2beaa08d467ff14a630 100644 Binary files a/assays/MetaboliteAnalysis/isa.assay.xlsx and b/assays/MetaboliteAnalysis/isa.assay.xlsx differ