From 1514c8d774066007741ac810267aa84dbe8fd927 Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Sat, 11 Jan 2025 14:35:33 +0100
Subject: [PATCH] add camalexin analysis protocol

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 assays/MetaboliteAnalysis/protocols/CamalexinAnalysis.md | 3 +++
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 create mode 100644 assays/MetaboliteAnalysis/protocols/CamalexinAnalysis.md

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+## Camalexin
+
+Camalexin was extracted from ~50 mg of leaves in 200 µl of dimethlysulfoxide (DMSO) as described in Koprivova et al. (2019). After centrifugation at room temperature for 20 min, 20 µl aliquots of the supernatant were injected into a Thermo Scientific Dionex UltiMate 3000 HPLC system with a Waters Spherisorb ODS-2 column (250 mm×4.6 mm, 5 µm). The samples were resolved using a gradient of acetonitrile in 0.01% (v/v) formic acid. Camalexin was detected by a fluorescence detector with an excitation at 318 nm and emission at 368 nm. For the quantification, external standards were used ranging from 1 pg µl–1 to 1 ng µl–1 (Koprivova et al., 2019).
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