diff --git a/assays/SulfateUptake/README.md b/assays/SulfateUptake/README.md new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/SulfateUptake/dataset/.gitkeep b/assays/SulfateUptake/dataset/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/SulfateUptake/isa.assay.xlsx b/assays/SulfateUptake/isa.assay.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..a97bdaabd0c82367dd667622363cd733eb754d67 Binary files /dev/null and b/assays/SulfateUptake/isa.assay.xlsx differ diff --git a/assays/SulfateUptake/protocols/.gitkeep b/assays/SulfateUptake/protocols/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/SulfateUptake/protocols/SulfateUptakeProtocol.md b/assays/SulfateUptake/protocols/SulfateUptakeProtocol.md new file mode 100644 index 0000000000000000000000000000000000000000..a384eeb681b2bd9708fb9bf9bf715cea98a53044 --- /dev/null +++ b/assays/SulfateUptake/protocols/SulfateUptakeProtocol.md @@ -0,0 +1,3 @@ +## Sulfate uptake + +Sulfate uptake was measured in seedlings grown for 2 weeks in 12-well plates on a modified Long-Ashton medium under S-sufficient or S-deficient conditions. For uptake measurement, the medium was exchanged with 1 ml of the Long-Ashton medium with 0.2 mM sulfate supplemented with 12 mCi of [35S]sulfuric acid and incubated for 30 min in the light. Whole seedlings still on the mesh were washed thoroughly, blotted dry, and shoot and root samples were cut, weighed, and stored separately in liquid nitrogen until further processing on the same day. Samples were extracted in a 10-fold volume of 0.1 M HCl. A 10 μl aliquot of extract was used to determine sulfate uptake by scintillation counting (Dietzen et al., 2020). \ No newline at end of file