From 6ba21ecdb683e26d8b7690370fc38554c6909775 Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Tue, 26 Nov 2024 17:43:48 +0100
Subject: [PATCH] create sulfate uptake assay and protocol

---
 assays/SulfateUptake/README.md                   |   0
 assays/SulfateUptake/dataset/.gitkeep            |   0
 assays/SulfateUptake/isa.assay.xlsx              | Bin 0 -> 6896 bytes
 assays/SulfateUptake/protocols/.gitkeep          |   0
 .../protocols/SulfateUptakeProtocol.md           |   3 +++
 5 files changed, 3 insertions(+)
 create mode 100644 assays/SulfateUptake/README.md
 create mode 100644 assays/SulfateUptake/dataset/.gitkeep
 create mode 100644 assays/SulfateUptake/isa.assay.xlsx
 create mode 100644 assays/SulfateUptake/protocols/.gitkeep
 create mode 100644 assays/SulfateUptake/protocols/SulfateUptakeProtocol.md

diff --git a/assays/SulfateUptake/README.md b/assays/SulfateUptake/README.md
new file mode 100644
index 0000000..e69de29
diff --git a/assays/SulfateUptake/dataset/.gitkeep b/assays/SulfateUptake/dataset/.gitkeep
new file mode 100644
index 0000000..e69de29
diff --git a/assays/SulfateUptake/isa.assay.xlsx b/assays/SulfateUptake/isa.assay.xlsx
new file mode 100644
index 0000000000000000000000000000000000000000..a97bdaabd0c82367dd667622363cd733eb754d67
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diff --git a/assays/SulfateUptake/protocols/.gitkeep b/assays/SulfateUptake/protocols/.gitkeep
new file mode 100644
index 0000000..e69de29
diff --git a/assays/SulfateUptake/protocols/SulfateUptakeProtocol.md b/assays/SulfateUptake/protocols/SulfateUptakeProtocol.md
new file mode 100644
index 0000000..a384eeb
--- /dev/null
+++ b/assays/SulfateUptake/protocols/SulfateUptakeProtocol.md
@@ -0,0 +1,3 @@
+## Sulfate uptake
+
+Sulfate uptake was measured in seedlings grown for 2 weeks in 12-well plates on a modified Long-Ashton medium under S-sufficient or S-deficient conditions. For uptake measurement, the medium was exchanged with 1 ml of the Long-Ashton medium with 0.2 mM sulfate supplemented with 12 mCi of [35S]sulfuric acid and incubated for 30 min in the light. Whole seedlings still on the mesh were washed thoroughly, blotted dry, and shoot and root samples were cut, weighed, and stored separately in liquid nitrogen until further processing on the same day. Samples were extracted in a 10-fold volume of 0.1 M HCl. A 10 μl aliquot of extract was used to determine sulfate uptake by scintillation counting (Dietzen et al., 2020).
\ No newline at end of file
-- 
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