diff --git a/assays/AssessmentOfPlantResistanceToPseudomonasSyringae/README.md b/assays/AssessmentOfPlantResistanceToPseudomonasSyringae/README.md new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/AssessmentOfPlantResistanceToPseudomonasSyringae/dataset/.gitkeep b/assays/AssessmentOfPlantResistanceToPseudomonasSyringae/dataset/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/AssessmentOfPlantResistanceToPseudomonasSyringae/isa.assay.xlsx b/assays/AssessmentOfPlantResistanceToPseudomonasSyringae/isa.assay.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..01b1294e4a36157e830d77f48ff0fbd409dca366 Binary files /dev/null and b/assays/AssessmentOfPlantResistanceToPseudomonasSyringae/isa.assay.xlsx differ diff --git a/assays/AssessmentOfPlantResistanceToPseudomonasSyringae/protocols/.gitkeep b/assays/AssessmentOfPlantResistanceToPseudomonasSyringae/protocols/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/AssessmentOfPlantResistanceToPseudomonasSyringae/protocols/AssessmentOfPlantResistanceToPseudomonasSyringaeProtocol.md b/assays/AssessmentOfPlantResistanceToPseudomonasSyringae/protocols/AssessmentOfPlantResistanceToPseudomonasSyringaeProtocol.md new file mode 100644 index 0000000000000000000000000000000000000000..ff2660ff4904015dcd4e7faa955beb9b4f57b806 --- /dev/null +++ b/assays/AssessmentOfPlantResistanceToPseudomonasSyringae/protocols/AssessmentOfPlantResistanceToPseudomonasSyringaeProtocol.md @@ -0,0 +1,3 @@ +## Assessment of plant resistance to *Pseudomonas syringae* + +To assess bacterial growth in naive 5-week-old plants, overnight log phase cultures of *Pseudomonas syringae* pathovar *maculicola* were washed three times with 10 mM MgCl2 and diluted to a final optical density at 600nm (OD600)=0.001 before infiltrating the resulting bacterial suspensions from the abaxial side into three fully grown leaves using a 1 ml syringe without a needle. The infiltration was performed between 10.00 h and 11.00 h, as previously described (DOI: 10.1016/j.cell.2018.02.049). Approximately 60 h later, the bacterial growth was quantified by measuring the bacterial bioluminescence in leaf discs (10 mm in diameter) of infiltrated leaves (one disc per leaf, three discs per plant) using a Sirius FB12 luminometer (Berthold Detection Systems). For each independent experiment, at least 18 replicate leaves from 6–7 plants per genotype were measured before performing a statistical analysis of the resulting values. \ No newline at end of file