From d42a46560e243b9f5d0b62b40e4e67b65d98c963 Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Sat, 11 Jan 2025 14:48:56 +0100
Subject: [PATCH] create plant resistance assay and add protocol
---
.../README.md | 0
.../dataset/.gitkeep | 0
.../isa.assay.xlsx | Bin 0 -> 6919 bytes
.../protocols/.gitkeep | 0
...antResistanceToPseudomonasSyringaeProtocol.md | 3 +++
5 files changed, 3 insertions(+)
create mode 100644 assays/AssessmentOfPlantResistanceToPseudomonasSyringae/README.md
create mode 100644 assays/AssessmentOfPlantResistanceToPseudomonasSyringae/dataset/.gitkeep
create mode 100644 assays/AssessmentOfPlantResistanceToPseudomonasSyringae/isa.assay.xlsx
create mode 100644 assays/AssessmentOfPlantResistanceToPseudomonasSyringae/protocols/.gitkeep
create mode 100644 assays/AssessmentOfPlantResistanceToPseudomonasSyringae/protocols/AssessmentOfPlantResistanceToPseudomonasSyringaeProtocol.md
diff --git a/assays/AssessmentOfPlantResistanceToPseudomonasSyringae/README.md b/assays/AssessmentOfPlantResistanceToPseudomonasSyringae/README.md
new file mode 100644
index 0000000..e69de29
diff --git a/assays/AssessmentOfPlantResistanceToPseudomonasSyringae/dataset/.gitkeep b/assays/AssessmentOfPlantResistanceToPseudomonasSyringae/dataset/.gitkeep
new file mode 100644
index 0000000..e69de29
diff --git a/assays/AssessmentOfPlantResistanceToPseudomonasSyringae/isa.assay.xlsx b/assays/AssessmentOfPlantResistanceToPseudomonasSyringae/isa.assay.xlsx
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diff --git a/assays/AssessmentOfPlantResistanceToPseudomonasSyringae/protocols/.gitkeep b/assays/AssessmentOfPlantResistanceToPseudomonasSyringae/protocols/.gitkeep
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index 0000000..e69de29
diff --git a/assays/AssessmentOfPlantResistanceToPseudomonasSyringae/protocols/AssessmentOfPlantResistanceToPseudomonasSyringaeProtocol.md b/assays/AssessmentOfPlantResistanceToPseudomonasSyringae/protocols/AssessmentOfPlantResistanceToPseudomonasSyringaeProtocol.md
new file mode 100644
index 0000000..ff2660f
--- /dev/null
+++ b/assays/AssessmentOfPlantResistanceToPseudomonasSyringae/protocols/AssessmentOfPlantResistanceToPseudomonasSyringaeProtocol.md
@@ -0,0 +1,3 @@
+## Assessment of plant resistance to *Pseudomonas syringae*
+
+To assess bacterial growth in naive 5-week-old plants, overnight log phase cultures of *Pseudomonas syringae* pathovar *maculicola* were washed three times with 10 mM MgCl2 and diluted to a final optical density at 600nm (OD600)=0.001 before infiltrating the resulting bacterial suspensions from the abaxial side into three fully grown leaves using a 1 ml syringe without a needle. The infiltration was performed between 10.00 h and 11.00 h, as previously described (DOI: 10.1016/j.cell.2018.02.049). Approximately 60 h later, the bacterial growth was quantified by measuring the bacterial bioluminescence in leaf discs (10 mm in diameter) of infiltrated leaves (one disc per leaf, three discs per plant) using a Sirius FB12 luminometer (Berthold Detection Systems). For each independent experiment, at least 18 replicate leaves from 6–7 plants per genotype were measured before performing a statistical analysis of the resulting values.
\ No newline at end of file
--
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