diff --git a/assays/Co-cultivationWithBurkholderiaGlumae/README.md b/assays/Co-cultivationWithBurkholderiaGlumae/README.md new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/Co-cultivationWithBurkholderiaGlumae/dataset/.gitkeep b/assays/Co-cultivationWithBurkholderiaGlumae/dataset/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/Co-cultivationWithBurkholderiaGlumae/isa.assay.xlsx b/assays/Co-cultivationWithBurkholderiaGlumae/isa.assay.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..115c3e2d1340309645401419f8c2eb25f1164194 Binary files /dev/null and b/assays/Co-cultivationWithBurkholderiaGlumae/isa.assay.xlsx differ diff --git a/assays/Co-cultivationWithBurkholderiaGlumae/protocols/.gitkeep b/assays/Co-cultivationWithBurkholderiaGlumae/protocols/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/Co-cultivationWithBurkholderiaGlumae/protocols/Co-cultivationWithBurkholderiaGlumaeProtocol.md b/assays/Co-cultivationWithBurkholderiaGlumae/protocols/Co-cultivationWithBurkholderiaGlumaeProtocol.md new file mode 100644 index 0000000000000000000000000000000000000000..95d0f5553729ec1b8617dbd02af763925e23d73b --- /dev/null +++ b/assays/Co-cultivationWithBurkholderiaGlumae/protocols/Co-cultivationWithBurkholderiaGlumaeProtocol.md @@ -0,0 +1,3 @@ +## Co-cultivation with *Burkholderia glumae* + +Plants were grown in the 12-well plates as described above for 14 d. For inoculation, overnight bacterial cultures were washed twice with sterile 10 mM MgCl2 and the final OD600 was measured. The *B. glumae* PG1 was diluted stepwise to OD600=0.0005 in 10 mM MgCl2. An 8 µl aliquot of these suspensions was used for inoculation into each well and 8 µl of 10 mM MgCl2 was used as mock treatment. Samples for camalexin (shoots) and DNA (roots) were harvested after 3 d of inoculation. \ No newline at end of file