From f586eb7ed0d51d1c6cd4d84b501479c1d22cb27d Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Sat, 11 Jan 2025 15:37:08 +0100
Subject: [PATCH] add co-cultivation assay and protocol

---
 .../README.md                                    |   0
 .../dataset/.gitkeep                             |   0
 .../isa.assay.xlsx                               | Bin 0 -> 6913 bytes
 .../protocols/.gitkeep                           |   0
 ...-cultivationWithBurkholderiaGlumaeProtocol.md |   3 +++
 5 files changed, 3 insertions(+)
 create mode 100644 assays/Co-cultivationWithBurkholderiaGlumae/README.md
 create mode 100644 assays/Co-cultivationWithBurkholderiaGlumae/dataset/.gitkeep
 create mode 100644 assays/Co-cultivationWithBurkholderiaGlumae/isa.assay.xlsx
 create mode 100644 assays/Co-cultivationWithBurkholderiaGlumae/protocols/.gitkeep
 create mode 100644 assays/Co-cultivationWithBurkholderiaGlumae/protocols/Co-cultivationWithBurkholderiaGlumaeProtocol.md

diff --git a/assays/Co-cultivationWithBurkholderiaGlumae/README.md b/assays/Co-cultivationWithBurkholderiaGlumae/README.md
new file mode 100644
index 0000000..e69de29
diff --git a/assays/Co-cultivationWithBurkholderiaGlumae/dataset/.gitkeep b/assays/Co-cultivationWithBurkholderiaGlumae/dataset/.gitkeep
new file mode 100644
index 0000000..e69de29
diff --git a/assays/Co-cultivationWithBurkholderiaGlumae/isa.assay.xlsx b/assays/Co-cultivationWithBurkholderiaGlumae/isa.assay.xlsx
new file mode 100644
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diff --git a/assays/Co-cultivationWithBurkholderiaGlumae/protocols/.gitkeep b/assays/Co-cultivationWithBurkholderiaGlumae/protocols/.gitkeep
new file mode 100644
index 0000000..e69de29
diff --git a/assays/Co-cultivationWithBurkholderiaGlumae/protocols/Co-cultivationWithBurkholderiaGlumaeProtocol.md b/assays/Co-cultivationWithBurkholderiaGlumae/protocols/Co-cultivationWithBurkholderiaGlumaeProtocol.md
new file mode 100644
index 0000000..95d0f55
--- /dev/null
+++ b/assays/Co-cultivationWithBurkholderiaGlumae/protocols/Co-cultivationWithBurkholderiaGlumaeProtocol.md
@@ -0,0 +1,3 @@
+## Co-cultivation with *Burkholderia glumae*
+
+Plants were grown in the 12-well plates as described above for 14 d. For inoculation, overnight bacterial cultures were washed twice with sterile 10 mM MgCl2 and the final OD600 was measured. The *B. glumae* PG1 was diluted stepwise to OD600=0.0005 in 10 mM MgCl2. An 8 µl aliquot of these suspensions was used for inoculation into each well and 8 µl of 10 mM MgCl2 was used as mock treatment. Samples for camalexin (shoots) and DNA (roots) were harvested after 3 d of inoculation.
\ No newline at end of file
-- 
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