From f586eb7ed0d51d1c6cd4d84b501479c1d22cb27d Mon Sep 17 00:00:00 2001 From: Viktoria Petrova <vipet103@hhu.de> Date: Sat, 11 Jan 2025 15:37:08 +0100 Subject: [PATCH] add co-cultivation assay and protocol --- .../README.md | 0 .../dataset/.gitkeep | 0 .../isa.assay.xlsx | Bin 0 -> 6913 bytes .../protocols/.gitkeep | 0 ...-cultivationWithBurkholderiaGlumaeProtocol.md | 3 +++ 5 files changed, 3 insertions(+) create mode 100644 assays/Co-cultivationWithBurkholderiaGlumae/README.md create mode 100644 assays/Co-cultivationWithBurkholderiaGlumae/dataset/.gitkeep create mode 100644 assays/Co-cultivationWithBurkholderiaGlumae/isa.assay.xlsx create mode 100644 assays/Co-cultivationWithBurkholderiaGlumae/protocols/.gitkeep create mode 100644 assays/Co-cultivationWithBurkholderiaGlumae/protocols/Co-cultivationWithBurkholderiaGlumaeProtocol.md diff --git a/assays/Co-cultivationWithBurkholderiaGlumae/README.md b/assays/Co-cultivationWithBurkholderiaGlumae/README.md new file mode 100644 index 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b/assays/Co-cultivationWithBurkholderiaGlumae/protocols/Co-cultivationWithBurkholderiaGlumaeProtocol.md new file mode 100644 index 0000000..95d0f55 --- /dev/null +++ b/assays/Co-cultivationWithBurkholderiaGlumae/protocols/Co-cultivationWithBurkholderiaGlumaeProtocol.md @@ -0,0 +1,3 @@ +## Co-cultivation with *Burkholderia glumae* + +Plants were grown in the 12-well plates as described above for 14 d. For inoculation, overnight bacterial cultures were washed twice with sterile 10 mM MgCl2 and the final OD600 was measured. The *B. glumae* PG1 was diluted stepwise to OD600=0.0005 in 10 mM MgCl2. An 8 µl aliquot of these suspensions was used for inoculation into each well and 8 µl of 10 mM MgCl2 was used as mock treatment. Samples for camalexin (shoots) and DNA (roots) were harvested after 3 d of inoculation. \ No newline at end of file -- GitLab