From 28cff6bf71df48e19f70e2cc96cd525fd14aaf50 Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Mon, 25 Nov 2024 13:38:36 +0100
Subject: [PATCH] add climate chamber experiment study and protocol
---
.../README.md | 0
.../isa.study.xlsx | Bin 0 -> 7517 bytes
.../protocols/.gitkeep | 0
...berExperimentalConditionsAndDesignProtocol.md | 3 +++
.../resources/.gitkeep | 0
5 files changed, 3 insertions(+)
create mode 100644 studies/ClimateChamberExperimentalConditionsAndDesign/README.md
create mode 100644 studies/ClimateChamberExperimentalConditionsAndDesign/isa.study.xlsx
create mode 100644 studies/ClimateChamberExperimentalConditionsAndDesign/protocols/.gitkeep
create mode 100644 studies/ClimateChamberExperimentalConditionsAndDesign/protocols/ClimateChamberExperimentalConditionsAndDesignProtocol.md
create mode 100644 studies/ClimateChamberExperimentalConditionsAndDesign/resources/.gitkeep
diff --git a/studies/ClimateChamberExperimentalConditionsAndDesign/README.md b/studies/ClimateChamberExperimentalConditionsAndDesign/README.md
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index 0000000..e69de29
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diff --git a/studies/ClimateChamberExperimentalConditionsAndDesign/protocols/.gitkeep b/studies/ClimateChamberExperimentalConditionsAndDesign/protocols/.gitkeep
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diff --git a/studies/ClimateChamberExperimentalConditionsAndDesign/protocols/ClimateChamberExperimentalConditionsAndDesignProtocol.md b/studies/ClimateChamberExperimentalConditionsAndDesign/protocols/ClimateChamberExperimentalConditionsAndDesignProtocol.md
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+++ b/studies/ClimateChamberExperimentalConditionsAndDesign/protocols/ClimateChamberExperimentalConditionsAndDesignProtocol.md
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+## Climate chamber experimental conditions and design
+
+Based on the results of the field experiments, six representative barley inbreds (HOR1842, IG128216, IG31424, ItuNative, K10877, and W23829/803911) were selected for a climate chamber experiment. The experimental design was a randomized complete block design with three replicates/blocks. A total of 32 plants were planted for each inbred in total. The gas exchange measurements were performed 21, 24, 26, 31, 36, 39, 46, 51, 57, 74, 87, 88, 100, and 102 days after sowing (DAS). At each measurement day, three measurements were taken from three different plants of an inbred, where always one plant from each of the blocks was studied. In addition, the Zadoks score for each evaluated plant was rated. In addition, we harvested three plants per inbred (i.e. one per block) and then measured dry weight of aboveground biomass per plant 26, 36, 46, 57, 74, 102, 113, and 142 DAS. The growth conditions in the climate chamber were as follows: 14 h/10 h light/dark photoperiod, 18 °C/16 °C temperature, and 55% relative humidity. The maximal light intensity measured at 15 cm from the light panel was 750 μmol m−2 s−1.
\ No newline at end of file
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