From bfffa373ed54ccd18d3a82dba241a1f3007b6f95 Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Mon, 25 Nov 2024 14:36:28 +0100
Subject: [PATCH] adjust all equations in protocol

---
 ...fMorphologicalAndGrowth-relatedTraitsProtocol.md | 13 ++++++-------
 1 file changed, 6 insertions(+), 7 deletions(-)

diff --git a/assays/AssessmentOfMorphologicalAndGrowth-relatedTraits/protocols/AssessmentOfMorphologicalAndGrowth-relatedTraitsProtocol.md b/assays/AssessmentOfMorphologicalAndGrowth-relatedTraits/protocols/AssessmentOfMorphologicalAndGrowth-relatedTraitsProtocol.md
index aa2dc6b..11915c2 100644
--- a/assays/AssessmentOfMorphologicalAndGrowth-relatedTraits/protocols/AssessmentOfMorphologicalAndGrowth-relatedTraitsProtocol.md
+++ b/assays/AssessmentOfMorphologicalAndGrowth-relatedTraits/protocols/AssessmentOfMorphologicalAndGrowth-relatedTraitsProtocol.md
@@ -16,17 +16,16 @@ To assess the relationship between DMP and time, logistic (Verhulst, 1838), powe
 **(2)**
 $$y_r=a+bt-ct^2$$
 
-where *a* represents the initial biomass, *b* and *c* the growth rate parameters. This model had a high coefficient of determination (⁠$$R^2$$⁠) and the highest heritability across all 23 barley inbreds. Thus, the quadratic regression was used for estimation of RGR. $$RGR_a$$, $$RGR_b$$, $$RGR_c$$ represent the parameters in quadratic regression *a*, *b*, and *c*, respectively.
+where *a* represents the initial biomass, *b* and *c* the growth rate parameters. This model had a high coefficient of determination (⁠$R^2$⁠) and the highest heritability across all 23 barley inbreds. Thus, the quadratic regression was used for estimation of RGR. $RGR_a$, $RGR_b$, $RGR_c$ represent the parameters in quadratic regression *a*, *b*, and *c*, respectively.
 
 Morphological parameters were collected in multi-year and multi-environment field experiments that took place in the years 2017–2021 at Düsseldorf, Cologne, Mechernich, and Quedlinburg (Shrestha et al., 2022; Wu et al., 2022). Not all locations were used in all years to assess all parameters. Flag leaf length (FL, cm) and width (FW, cm), plant height (PH, cm), flowering time (FT), awn length (AL, cm), spike length (EL, cm), and spikelet number in one row of the spike (SR), seed length (SL, mm), seed width (SW, mm), seed area (SA, mm2), and thousand grain weight (TGW, g), grain weight (GW, kg per 10 m2), and net straw weight (NSW, kg per 10 m2) were measured as morphological parameters. FL, FW, AL, EL were measured by ruler, SL, SW, and SA were measured by MARViN seed analyser (MARViNTECH GmbH, Germany), and TGW was measured by MARViN and a balance.
 
 The same set of morphological parameters were also measured in the climate chamber experiment. FL and FW were collected at 74 and 102 DAS with three replicates, and spike-related traits (AL, EL, SR, SL, SW SA, and TGW) were collected at 142 DAS with three replicates. Additionally, the total stem (without spike) weight per plant (SWP, g), total spike weight per plant (SKWP, g), total stem weight of main stem (TSWM, g), and spike weight of main stem (SKWM, g) were also collected in the climate chamber experiment. Harvest index (HI) was calculated using the following equation:
 
- 
- 
-(3)
+**(3)**
+$$HI=\frac{SKWP}{DMP}$$
+
 In addition, harvest index of main stem (MSHI) was calculated using the following equation:
 
- 
- 
-(4)
\ No newline at end of file
+**(4)**
+$$MSHI=\frac{SKWP}{TSWM}$$
\ No newline at end of file
-- 
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