From f7fa497c81512142a09450b3c86d3f5f6c2f042a Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Tue, 26 Nov 2024 16:41:03 +0100
Subject: [PATCH] add climate chamber experiment protocol

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 .../protocols/ClimateChamberExperiment.md                | 9 +++++++++
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 create mode 100644 assays/StatisticalAnalysis/protocols/ClimateChamberExperiment.md

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+## Climate chamber experiment
+
+The adjusted entry means of carbon assimilation-related parameters from the climate chamber experiment were calculated based on the following model:
+ 
+**(7)**
+$$y_{(A)ijklmr} = \mu + G_i + ZS_j + D_k + (D:TW)_{kl} + S_m + (G:S)_{im} + \epsilon_{(A)ijklmr}$$
+where $y_{(A)ijklmr}$ was the carbon assimilation-related parameter, $(D:TW)_{kl}$ the effect of the *l*th time window in the *k*th date of measurement, and $\epsilon_{(A)ijklmr}$ the random error. To estimate adjusted entry means for carbon assimilation-related parameters of six barley inbreds, $G_i, ZS_j, S_m$ and $(G:S)_{im}$ were treated as fixed effects, as well as $D_k$ and $(D:TW)_{kl}$ as random effects.
+
+The relationship between photosynthesis-related parameters and morphological or growth-related parameters of the inbreds was evaluated by Pearson’s correlation coefficient between adjusted entry means.
\ No newline at end of file
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