<img src=dataset\fpls-14-1024981-g002.jpg width=60%>

### Figure 2 caption

**Figure 2** Squalene concentrations [mg l-1 OD750-1] in response to gene overexpressions. Values are represented as the means of three biological replicates, standard deviations are shown. WT represents the *Synechocystis* sp. PCC 6803 wild type, while the control strain is *Synechocystis* sp. PCC 6803 Δshc pSHDY rhaS, from which the overexpression strains were constructed by inserting an additional copy of the specified gene under the control of the rhamnose-inducible promoter Prha into its genome. Asterisks ( * ) represent the p-value of the two-sided t-test between the respective strain and the control strain at the same rhamnose concentration (* denotes a value of p<0.05, ** denotes p<0.01 and *** denotes p<0.001). Samples were measured after three days of incubation with the specified concentration of rhamnose as an inducer.

### Figure 2 source

https://www.frontiersin.org/files/Articles/1024981/fpls-14-1024981-HTML/image_m/fpls-14-1024981-g002.jpg

<img src=dataset\fpls-14-1024981-g005.jpg width=60%>

### Figure 5 caption

**Figure 5** Timeseries of squalene production in sqs overexpression strain. Squalene production and OD750 of *Synechocystis* Δshc pEERM Prha sqs pSHDY rhaS in a 30 ml flask culture in mg l-1 over a period of two weeks after induction with 5 mM rhamnose to trigger overexpression of the squalene synthase (sqs). Means and standard deviations of three biological replicates are shown.

### Figure 5 source

https://www.frontiersin.org/files/Articles/1024981/fpls-14-1024981-HTML/image_m/fpls-14-1024981-g005.jpg

<img src=dataset\SupplementaryFigure2.jpg width=60%>
### Supplementary Figure 2 caption

**Supplementary Figure 2.** GC-MS calibration curve for squalene after extraction of 50, 25, 12.5,
6.25 and 3.125 µM of squalene using the method for squalene extraction from Synechocystis cells.
Relative response is in relation to the 25 µM β-sitosterol standard, which was solved in the acetone
used for extraction.

### Supplementary Figure 2 source

https://www.frontiersin.org/articles/10.3389/fpls.2023.1024981/full#supplementary-material