diff --git a/assays/cPCR/README.md b/assays/cPCR/README.md index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..8847e03d3d44ab95fdb5ff74045ec65cdfcd572b 100644 --- a/assays/cPCR/README.md +++ b/assays/cPCR/README.md @@ -0,0 +1,14 @@ +<img src=protocols\SupplementaryFigure1.jpg width=60%> + +### Supplementary Figure 1 caption + +**Supplementary Figure 1.** Agarose gel electrophoresis of colony PCR products to prove the +integration of the respective gene into the genome through heterologous recombination into the +neutral site 2 (NS2). Number denote tested colonies, sizes of the expected PCR bands are shown. +PCR was carried out with the colony PCR primers shown in Supplementary Table 2 (A) Dxs: 2232 +bp, IspD: 1002 bp, IspF: 795 bp (B) Tpi: 2041 bp, gapDH: 1323 bp, IspE: 1257 bp (C) IspG: 1521 bp +(D) IspH: 1401 bp, CrtE M87F: 1089 bp (E) PyrK:2085 bp (F) Sqs: 1119 bp, Ipi: 1359 bp + +### Supplementary Figure 1 source + +https://www.frontiersin.org/articles/10.3389/fpls.2023.1024981/full#supplementary-material \ No newline at end of file diff --git a/assays/cPCR/dataset/SupplementaryFigure1.jpg b/assays/cPCR/dataset/SupplementaryFigure1.jpg new file mode 100644 index 0000000000000000000000000000000000000000..c067e856795df5ed58d9fdc27e6757b2903d90ea --- /dev/null +++ b/assays/cPCR/dataset/SupplementaryFigure1.jpg @@ -0,0 +1,3 @@ +version https://git-lfs.github.com/spec/v1 +oid sha256:68641d4a69b7e30dcbecc54c8e3d6af42f09453475e89397ce4fb6e0b15aada5 +size 54167 diff --git a/assays/cPCR/protocols/TableS2_cPCR.xlsx b/assays/cPCR/protocols/TableS2_cPCR.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..5cebed488f4cd63f828646ec9a792ab362d49e81 Binary files /dev/null and b/assays/cPCR/protocols/TableS2_cPCR.xlsx differ diff --git a/assays/qPCR/protocols/TableS2_qPCR.xlsx b/assays/qPCR/protocols/TableS2_qPCR.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..88428921d1e772bae51bbbf4757527739dac737b Binary files /dev/null and b/assays/qPCR/protocols/TableS2_qPCR.xlsx differ diff --git a/studies/PlasmidAndStrainConstruction/protocols/SupplementaryFigure1.jpg b/studies/PlasmidAndStrainConstruction/protocols/SupplementaryFigure1.jpg deleted file mode 100644 index 7b13cc96f88158147aed3a0cdc3cd1451f3feddf..0000000000000000000000000000000000000000 Binary files a/studies/PlasmidAndStrainConstruction/protocols/SupplementaryFigure1.jpg and /dev/null differ diff --git a/studies/PlasmidAndStrainConstruction/protocols/TableS2.xlsx b/studies/PlasmidAndStrainConstruction/protocols/TableS2.xlsx index 3025d524fc5af59a7ac4f8a92b48dd676e49c676..9ab7c7d9b323bf8f30c3c7d5120edeeffe78ea0e 100644 Binary files a/studies/PlasmidAndStrainConstruction/protocols/TableS2.xlsx and b/studies/PlasmidAndStrainConstruction/protocols/TableS2.xlsx differ