A systematic overexpression approach reveals native targets to increase squalene production in Synechocystis sp. PCC 6803
Original publication
Germann AT, Nakielski A, Dietsch M, Petzel T, Moser D, Triesch S, Westhoff P and Axmann IM (2023) A systematic overexpression approach reveals native targets to increase squalene production in Synechocystis sp. PCC 6803. Front. Plant Sci. 14:1024981. doi: 10.3389/fpls.2023.1024981
Copyright
Copyright © 2023 Germann, Nakielski, Dietsch, Petzel, Moser, Triesch, Westhoff and Axmann. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
ARC structure
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subgraph study\Synechocystis
s1(Synechocystis mutants)---pr1>growth]-->cl1(mutant cultures)
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subgraph study\Englund-2014
nz1(Pia Lindberg)---pr9>gift]-->nz2(plasmid pEERM4 Cm)
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subgraph study\PlasmidAndStrainConstruction
nz2---pr10>cloning of genes]-->s2(Plasmid Constructs)
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subgraph study\KnoopAndSteuer-2015
nz4(Ralf Steuer)---pr15>send]-->nz5(extended version of a genome-scale stoichiometric network model of Synechocystis)
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subgraph assay\DCWMeasurements
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d1-->|normalization|a2
d2-->|normalization|a2
subgraph assay\GC-MSMeasurementsForTheQuantificationOfSqualene
cl1---pr4>GC-MS measurement]-->a1(Quantification of Squalene)
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subgraph assay\qPCR
cl1---pr6>RNA extraction]-->a3(RNA)---pr7>cDNA synthesis]-->a4(cDNA)---pr8>qRT-PCR]-->a5(qRT-PCR results)
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s1---pr13>cPCR]-->a6(cPCR results)---pr14>gel electrophoresis]-->a7(gel results)
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nz5---pr16>metabolic modeling and simulations]-->a8(amplification targets)---pr10
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