
@article{getzke_cofunctioning_2023,
	title = {Cofunctioning of bacterial exometabolites drives root microbiota establishment},
	volume = {120},
	issn = {0027-8424, 1091-6490},
	url = {https://pnas.org/doi/10.1073/pnas.2221508120},
	doi = {10.1073/pnas.2221508120},
	abstract = {Soil-dwelling microbes are the principal inoculum for the root microbiota, but our understanding of microbe–microbe interactions in microbiota establishment remains fragmentary. We tested 39,204 binary interbacterial interactions for inhibitory activities in vitro, allowing us to identify taxonomic signatures in bacterial inhibition profiles. Using genetic and metabolomic approaches, we identified the antimicrobial 2,4-diacetylphloroglucinol (DAPG) and the iron chelator pyoverdine as exometabolites whose combined functions explain most of the inhibitory activity of the strongly antagonistic
              Pseudomonas brassicacearum
              R401. Microbiota reconstitution with a core of
              Arabidopsis thaliana
              root commensals in the presence of wild-type or mutant strains revealed a root niche-specific cofunction of these exometabolites as root competence determinants and drivers of predictable changes in the root-associated community. In natural environments, both the corresponding biosynthetic operons are enriched in roots, a pattern likely linked to their role as iron sinks, indicating that these cofunctioning exometabolites are adaptive traits contributing to pseudomonad pervasiveness throughout the root microbiota.},
	language = {en},
	number = {15},
	urldate = {2024-06-21},
	journal = {Proceedings of the National Academy of Sciences},
	author = {Getzke, Felix and Hassani, M. Amine and Crüsemann, Max and Malisic, Milena and Zhang, Pengfan and Ishigaki, Yuji and Böhringer, Nils and Jiménez Fernández, Alicia and Wang, Lei and Ordon, Jana and Ma, Ka-Wai and Thiergart, Thorsten and Harbort, Christopher J. and Wesseler, Hidde and Miyauchi, Shingo and Garrido-Oter, Ruben and Shirasu, Ken and Schäberle, Till F. and Hacquard, Stéphane and Schulze-Lefert, Paul},
	month = apr,
	year = {2023},
	pages = {e2221508120},
	file = {Full Text:files/20808/Getzke et al. - 2023 - Cofunctioning of bacterial exometabolites drives r.pdf:application/pdf},
}
