diff --git a/.gitattributes b/.gitattributes index 50a354a3478b772addd449721b3ddd645dfc0bdc..e09f5624751fd10effd96d81a926b8450b4a3f6f 100644 --- a/.gitattributes +++ b/.gitattributes @@ -12,3 +12,4 @@ assays/ComplementationR401pvdY/dataset/.gitkeep filter=lfs diff=lfs merge=lfs -t assays/InvitroIronMobilizationAssay/dataset/.gitkeep filter=lfs diff=lfs merge=lfs -text assays/BacterialGrowthRates/dataset/.gitkeep filter=lfs diff=lfs merge=lfs -text assays/MicrobiotaReconstitutionFlowpot/dataset/.gitkeep filter=lfs diff=lfs merge=lfs -text +assays/Mono-association[[:space:]]experiment[[:space:]]of[[:space:]]R401[[:space:]]on[[:space:]]Athaliana[[:space:]]seedlings[[:space:]]in[[:space:]]agar[[:space:]]plates/dataset/.gitkeep filter=lfs diff=lfs merge=lfs -text diff --git a/assays/BacterialGrowthRates/isa.assay.xlsx b/assays/BacterialGrowthRates/isa.assay.xlsx index 371b4972d49c6239a81b46b7c58e62ef5735cbba..3320f2f3d0d71b8fae65f9293c871810b6cf3a3d 100644 Binary files a/assays/BacterialGrowthRates/isa.assay.xlsx and b/assays/BacterialGrowthRates/isa.assay.xlsx differ diff --git a/assays/Mono-association experiment of R401 on Athaliana seedlings in agar plates/README.md b/assays/Mono-association experiment of R401 on Athaliana seedlings in agar plates/README.md new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/Mono-association experiment of R401 on Athaliana seedlings in agar plates/dataset/.gitkeep b/assays/Mono-association experiment of R401 on Athaliana seedlings in agar plates/dataset/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/Mono-association experiment of R401 on Athaliana seedlings in agar plates/isa.assay.xlsx b/assays/Mono-association experiment of R401 on Athaliana seedlings in agar plates/isa.assay.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..6ad24632ec81f319efea8632dd09f40fc8214a9f Binary files /dev/null and b/assays/Mono-association experiment of R401 on Athaliana seedlings in agar plates/isa.assay.xlsx differ diff --git a/assays/Mono-association experiment of R401 on Athaliana seedlings in agar plates/protocols/.gitkeep b/assays/Mono-association experiment of R401 on Athaliana seedlings in agar plates/protocols/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/Mono-association experiment of R401 on Athaliana seedlings in agar plates/protocols/R401onAthalianaSeedlingsAgarPlates.md b/assays/Mono-association experiment of R401 on Athaliana seedlings in agar plates/protocols/R401onAthalianaSeedlingsAgarPlates.md new file mode 100644 index 0000000000000000000000000000000000000000..c7878ba34b7485ec952adf818719bccc87e07b74 --- /dev/null +++ b/assays/Mono-association experiment of R401 on Athaliana seedlings in agar plates/protocols/R401onAthalianaSeedlingsAgarPlates.md @@ -0,0 +1,3 @@ +## Mono-association experiment of R401 on A. thaliana seedlings in agar plates + +This protocol is adapted from (25). In brief, *A. thaliana* seeds were sterilized, germinated, and transferred to 1â„2 MS agar plates without sucrose, as described before. After transfer of seedlings, plants were grown for another 14 days under the same conditions. R401 wild-type and mutants were grown in 50% TSB overnight as described before. Bacterial cells were pelleted by centrifugation, washed 3x in 10 mM MgCl2 and OD~600~ was measured and adjusted to 0.0001. Agar plates were flushed with 15 ml of bacterial suspension for 5 min. The bacterial suspension were removed, and plants were carefully transferred to new 1â„2 MS agar plates. After 24 h, roots were cut using a sterile scalpel and collected in pre-weighed, sterile 2 ml tubes containing 1 steel bead (3 mm diameter). Tubes were weighed again to assess the root fresh weight. Subsequently, roots were ground in a Precellys 24 TissueLyser (Bertin Technologies) for 2 x 30 s at 6,200 rpm at 15 s intervals. Then, 250 μl of sterile 10 mM MgCl2 were added to each tube and roots were ground again under the same conditions. Each sample was subsequently 5x 1:10 diluted in sterile 10 mM MgCl2. Undiluted samples and each dilution were plated on 50% TSA square plates, dried and left to grow at 25 °C until single colonies appeared. Pictures were taken and single colonies were counted blinded. \ No newline at end of file