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@@ -10,3 +10,4 @@ assays/Mini-Tn5ForPyoverdineLackofR569/dataset/.gitkeep filter=lfs diff=lfs merg
 assays/Mini-Tn5IdentificationofIntegrationSitesR401andR569/dataset/.gitkeep filter=lfs diff=lfs merge=lfs -text
 assays/ComplementationR401pvdY/dataset/.gitkeep filter=lfs diff=lfs merge=lfs -text
 assays/InvitroIronMobilizationAssay/dataset/.gitkeep filter=lfs diff=lfs merge=lfs -text
+assays/BacterialGrowthRates/dataset/.gitkeep filter=lfs diff=lfs merge=lfs -text
diff --git a/assays/BacterialGrowthRates/README.md b/assays/BacterialGrowthRates/README.md
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+## Validation of bacterial growth rates
+
+The growth of individual R401 mutants and wild type was assessed by continuously measuring OD~600~ of actively growing bacterial cultures in an Infinite 200 PRO plate reader (TECAN) over 48 h at 25 °C and approx. 300 rpm. Overnight cultures in 50% TSB were pelleted and washed as described before. Subsequently, OD~600~ was measured and set to 0.02 in either artificial root exudates (ARE) or ARE lacking FeCl~3~. Composition of ARE
+was adopted from (21) and can be found in **Table S5**.
+
+(21) E. Baudoin, E. Benizri, A. Guckert, Impact of artificial root exudates on the bacterial community structure in bulk soil and maize rhizosphere. *Soil Biol Biochem* **35**, 1183–1192 (2003).
\ No newline at end of file
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