From 95ca08ee29431d6f8a30fbaeec3ef633cd2a6ebf Mon Sep 17 00:00:00 2001 From: Mekhrona Mirzoeva <memir101@hhu.de> Date: Thu, 13 Mar 2025 15:47:59 +0100 Subject: [PATCH] R401pvdY Complementation assay and protocol --- .gitattributes | 1 + assays/ComplementationR401pvdY/README.md | 0 assays/ComplementationR401pvdY/dataset/.gitkeep | 0 assays/ComplementationR401pvdY/isa.assay.xlsx | Bin 0 -> 9425 bytes .../ComplementationR401pvdY/protocols/.gitkeep | 0 .../ComplementationR401\342\210\206pvdy.md" | 5 +++++ 6 files changed, 6 insertions(+) create mode 100644 assays/ComplementationR401pvdY/README.md create mode 100644 assays/ComplementationR401pvdY/dataset/.gitkeep create mode 100644 assays/ComplementationR401pvdY/isa.assay.xlsx create mode 100644 assays/ComplementationR401pvdY/protocols/.gitkeep create mode 100644 "assays/ComplementationR401pvdY/protocols/ComplementationR401\342\210\206pvdy.md" diff --git a/.gitattributes b/.gitattributes index d99cf23..fb6ffed 100644 --- a/.gitattributes +++ b/.gitattributes @@ -8,3 +8,4 @@ assays/Mini-Tn5TransposonMutantInR401andR569/dataset/.gitkeep filter=lfs diff=lf assays/Mini-Tn5ScreenLossOfR401sGrowthInhibitionRsGMI1600/dataset/.gitkeep filter=lfs diff=lfs merge=lfs -text assays/Mini-Tn5ForPyoverdineLackofR569/dataset/.gitkeep filter=lfs diff=lfs merge=lfs -text assays/Mini-Tn5IdentificationofIntegrationSitesR401andR569/dataset/.gitkeep filter=lfs diff=lfs merge=lfs -text +assays/ComplementationR401pvdY/dataset/.gitkeep filter=lfs diff=lfs merge=lfs -text diff --git a/assays/ComplementationR401pvdY/README.md b/assays/ComplementationR401pvdY/README.md new file mode 100644 index 0000000..e69de29 diff --git a/assays/ComplementationR401pvdY/dataset/.gitkeep b/assays/ComplementationR401pvdY/dataset/.gitkeep new file mode 100644 index 0000000..e69de29 diff --git a/assays/ComplementationR401pvdY/isa.assay.xlsx b/assays/ComplementationR401pvdY/isa.assay.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..981f97cfbe1278262d6f3099df057f033f4c56d6 GIT binary patch literal 9425 zcmahvbzGEL*GPkOcQ*q_BZ8!KcXtg4gEUgo-Q6H1NOyxE(hVXdNOwv|euJ#5tGn-a z|CpKIGbipneb0Fmq@kb@As`^&A@&`uwUCF_sVpBrK%B!sKwyHOYKwqu987H-^i^DK zP3?7=U96vXsr1`)JVI+eW2DemG+I<p#r75fMlvaF!T}BgG<a%FQ>PZ3jp>RQ$b?0= zsNa67Vz07t6OYv`i9zON2_Kp!+pdCkH1wP3PxBaD7^6)%htPV(MqpnnK1{P5IO+}> zrHw207%D>BVP9{3Qx2PnADZGMwQLFhbvt2BxWb3}$gu6U=V1=iR#ZTMZ@9QJz_3L4 zs4-{N7d^1AYGoDTd9@wvM+Qy7ERWXFrAVolDm`@UM()%l`{=I@s}Ki=Oudai!#RGC z^zKE{aS2ROa4$IjSU=g3r0SHu5+<#jP$_5_;wVd>T!$ibj>tN}5X$;iXiKlj6WWcW zGs~Eg_J+^{>9$uq%PlZ)|GFfIgURRV4)#T9q(Dq9z51epJFGZrwLMf@!>ks{LpC>f zUHvwRm#dpLdn!3sg}zLxu7!d=LunNmV)31`Z!eRd$vRmE!|O&(MV{tLOgPtJrs^SJ znOD0K%`Jlz{!$IZch!Kk@%MrS|I)WJeQwY41Lj_RN12i{o8bSXAt4|h{td%?2U!yH zPOkG2lHk7kvz5yf8wvRAye6{Tc^ZzV?Nl~|HQ_n$efRc*qXdz{fp8HocGk`Nw+H#c zx5AZ9iI4zq39w-LhXRM%`)`0RP#GS=22#SY+2$gpu9;oSP)Zf{wnS=TOZ33Lj6TnD ztmT(YVvNA*lkW!#(+^K<2067yd@U;7cev<%wdPk?J0v*MNd4xTb&T`5jW%dAde`tZ znYq?gADxK|aj>`R#C}?U`dT)F!pOR=+EmjOe?f-CNOo3jngzOYq1;lxR+}TytS-X( zaG11k-E9L=ioXw~vnGP^726ms_lP^h4~hIKH7Kw|T%P|evOfV3?g9QyvNx^KF^Ay4 z#lS=eeg$&|*;yHZKvs9I@m<g@F@u(!kI?+~JrFC-v}!L!=%elF{dKg@9{APN$~>2} zs1xwKEJC&>g3pH=k4?gUa?CnrcFBsegiLwznVzna2)gk@>Ssqpy{+#4Cur|y@cCT= z5oxGCso|#55^)p|S9*{|%Mj6*4}8&+vIIn=u#t}BrGD&Iw2!HX72<ZA&juV{seNdG zQ=!c;GRJzKPA@p2+}`GLS-VT<elWNl6frC+u>_R3di}9NM9p?Y^XP%=(^eh!jB#d? 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Plasmid construction was conducted using Gibson assembly as described before. In brief, the coding region of R401 *pvdY* was co amplified with a 1.5 kb region upstream of the gene and ligated into a linearized pSEVA22l (18) vector backbone. This vector was integrated into *E. coli* BW29427 cells and subsequently integrated into R401 *∆pvdy via* bi-parental conjugation. + +(18) E. MartÃnez-GarcÃa, *et al.*, SEVA 3.0: an update of the Standard European Vector Architecture for enabling portability of genetic constructs among diverse bacterial hosts. *Nucleic Acids Res* **48**, D1164–D1170 (2020). \ No newline at end of file -- GitLab