diff --git a/assays/DetectionOfR401DAPGandPyoverdine/README.md b/assays/DetectionOfR401DAPGandPyoverdine/README.md new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/DetectionOfR401DAPGandPyoverdine/dataset/.gitkeep b/assays/DetectionOfR401DAPGandPyoverdine/dataset/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/DetectionOfR401DAPGandPyoverdine/isa.assay.xlsx b/assays/DetectionOfR401DAPGandPyoverdine/isa.assay.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..83487b87a3e8f49ae1100a8bc1d4c3397c6b73ef Binary files /dev/null and b/assays/DetectionOfR401DAPGandPyoverdine/isa.assay.xlsx differ diff --git a/assays/DetectionOfR401DAPGandPyoverdine/protocols/.gitkeep b/assays/DetectionOfR401DAPGandPyoverdine/protocols/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/DetectionOfR401DAPGandPyoverdine/protocols/DAPGandPyoverdineDetection.md b/assays/DetectionOfR401DAPGandPyoverdine/protocols/DAPGandPyoverdineDetection.md new file mode 100644 index 0000000000000000000000000000000000000000..b81ba3aa8b90f2bfde45a382f268cdb2ea2a5626 --- /dev/null +++ b/assays/DetectionOfR401DAPGandPyoverdine/protocols/DAPGandPyoverdineDetection.md @@ -0,0 +1,3 @@ +## Detection of R401 DAPG and pyoverdine + +For the detection of iron-chelating compounds, a R401 preculture was grown over night in 5 ml LB medium at 30 °C and 200 rpm. Before inoculation of the main culture, cells were washed twice with the main culture medium to remove potential traces of iron from the medium. Erlenmeyer flasks containing 20 ml of modified MM63 (KH2PO4 13.61 g/L, KOH 4.21 g/L, (NH4)SO4 1.98 g/L, MgSO4*7 H2O 0.25 g/L, NaCl 0.5g/L, Glucose * H2O 5,00 g/L, pH 7.1 KOH/HCl) with or without addition of 0.0011 g/L FeSO4 * 7 H2O were inoculated with 100 μl of preculture and cultivated for 6 days at 30 °C and 200 rpm. Every second day, 0.5 ml sample were taken, cells were removed by centrifugation and 5 μl of supernatant were analysed on a Bruker microTOFq-II high-resolution mass spectrometer coupled to an Agilent 1290 UPLC system with an Acquity UPLC BEH C-18 reverse phase column, run in a gradient of MeCN/H2O + 0.1% formic acid. Higher accuracy measurements were performed on a maXis-II qTOF, coupled to an identical LC setup as described earlier. \ No newline at end of file diff --git a/assays/MetabolomicAnalysis/isa.assay.xlsx b/assays/MetabolomicAnalysis/isa.assay.xlsx index f9c134cac112875e9feb7fb8879a86c6e50a9ad0..fde9a26ae419ad5beabc8193efba993c8c784dab 100644 Binary files a/assays/MetabolomicAnalysis/isa.assay.xlsx and b/assays/MetabolomicAnalysis/isa.assay.xlsx differ diff --git a/assays/Screen for antagonistic interbacterial interactions/isa.assay.xlsx b/assays/Screen for antagonistic interbacterial interactions/isa.assay.xlsx index d551349c89371bbe071c25f516a530e9371ea985..7b1eac32a44c5ac4a1db49f25ca84aa1e1c90859 100644 Binary files a/assays/Screen for antagonistic interbacterial interactions/isa.assay.xlsx and b/assays/Screen for antagonistic interbacterial interactions/isa.assay.xlsx differ diff --git a/isa.investigation.xlsx b/isa.investigation.xlsx index 7f49926976893ca51466264cda2e2a43bcd25bef..2a088ed43ac0fcf0a890e7ad6c02ebbf8b78ca1c 100644 Binary files a/isa.investigation.xlsx and b/isa.investigation.xlsx differ diff --git a/studies/Bacterial culture conditions/isa.study.xlsx b/studies/Bacterial culture conditions/isa.study.xlsx index 370d6099ad8a743fa399232e8f79d7ad2c409dae..96ce5007ccd6dc34aa7aa4bac652723ef03e3027 100644 Binary files a/studies/Bacterial culture conditions/isa.study.xlsx and b/studies/Bacterial culture conditions/isa.study.xlsx differ diff --git a/studies/Plant Growth Conditions/isa.study.xlsx b/studies/Plant Growth Conditions/isa.study.xlsx index 22e25ca925579e857d6a956701dca77f9b616d98..893340bebb6e28a606e2c5b1a66bcf80d82e49db 100644 Binary files a/studies/Plant Growth Conditions/isa.study.xlsx and b/studies/Plant Growth Conditions/isa.study.xlsx differ