From 2ad480421d6b7ea47e479d19559d7256b9668fbc Mon Sep 17 00:00:00 2001 From: Viktoria Petrova <vipet103@hhu.de> Date: Sat, 12 Oct 2024 20:45:55 +0200 Subject: [PATCH] add comparison of autophagic activity assay and protocol --- .../README.md | 0 .../dataset/.gitkeep | 0 .../isa.assay.xlsx | Bin 0 -> 6917 bytes .../protocols/.gitkeep | 0 ...Comparison-Of-Autophagic-Activity-In-Roots.md | 3 +++ 5 files changed, 3 insertions(+) create mode 100644 assays/ComparisonOfAutophagicActivityInRoots/README.md create mode 100644 assays/ComparisonOfAutophagicActivityInRoots/dataset/.gitkeep create mode 100644 assays/ComparisonOfAutophagicActivityInRoots/isa.assay.xlsx create mode 100644 assays/ComparisonOfAutophagicActivityInRoots/protocols/.gitkeep create mode 100644 assays/ComparisonOfAutophagicActivityInRoots/protocols/Comparison-Of-Autophagic-Activity-In-Roots.md diff --git a/assays/ComparisonOfAutophagicActivityInRoots/README.md b/assays/ComparisonOfAutophagicActivityInRoots/README.md new file 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b/assays/ComparisonOfAutophagicActivityInRoots/protocols/Comparison-Of-Autophagic-Activity-In-Roots.md new file mode 100644 index 0000000..4deef55 --- /dev/null +++ b/assays/ComparisonOfAutophagicActivityInRoots/protocols/Comparison-Of-Autophagic-Activity-In-Roots.md @@ -0,0 +1,3 @@ +## Comparison of autophagic activity in roots mounted on glass or in RoPods + +To assess the impact of glass-based sample mounting on the autophagic activity, RoPod v24 and standard agar plates were prepared similarly to the experiment for the root length measurement. After 6 days, seedlings growing on top of the agar medium were transferred in an empty glass bottom chamber, on a drop of 0.5 × MS and then gently covered with a cover slide. To prevent fast evaporation of the liquid medium, the glass bottom chamber was covered with a lid. Both RoPod v24 with seedlings and glass bottom chamber with transferred seedlings were imaged at different end points using CLSM 800 (Carl Zeiss), Objective 40x/1.2W, excitation light 488 nm, and emission 505–560 nm. The number of puncta was quantified in *Fiji* using the workflow presented in Supplementary File S2. \ No newline at end of file -- GitLab