From 6a4dfffe1206b2db562980660dbf6d711003fda2 Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Sat, 12 Oct 2024 13:34:45 +0200
Subject: [PATCH] add plant material study and protocol

---
 studies/PlantMaterial/README.md                  |   0
 studies/PlantMaterial/isa.study.xlsx             | Bin 0 -> 7500 bytes
 studies/PlantMaterial/protocols/.gitkeep         |   0
 .../PlantMaterial/protocols/Plant-Material.md    |   9 +++++++++
 studies/PlantMaterial/resources/.gitkeep         |   0
 5 files changed, 9 insertions(+)
 create mode 100644 studies/PlantMaterial/README.md
 create mode 100644 studies/PlantMaterial/isa.study.xlsx
 create mode 100644 studies/PlantMaterial/protocols/.gitkeep
 create mode 100644 studies/PlantMaterial/protocols/Plant-Material.md
 create mode 100644 studies/PlantMaterial/resources/.gitkeep

diff --git a/studies/PlantMaterial/README.md b/studies/PlantMaterial/README.md
new file mode 100644
index 0000000..e69de29
diff --git a/studies/PlantMaterial/isa.study.xlsx b/studies/PlantMaterial/isa.study.xlsx
new file mode 100644
index 0000000000000000000000000000000000000000..843799646b44e83c43af6f37212144c54c94bf47
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diff --git a/studies/PlantMaterial/protocols/.gitkeep b/studies/PlantMaterial/protocols/.gitkeep
new file mode 100644
index 0000000..e69de29
diff --git a/studies/PlantMaterial/protocols/Plant-Material.md b/studies/PlantMaterial/protocols/Plant-Material.md
new file mode 100644
index 0000000..fafe3d1
--- /dev/null
+++ b/studies/PlantMaterial/protocols/Plant-Material.md
@@ -0,0 +1,9 @@
+## Plant Material
+
+Autophagy deficient *atg5/atg7* double knockout line was established by crossing previously published *atg5-1* (Thomson et al., 2005) and *atg7-2* (Hofius, D. et al., 2009) mutants.
+
+Col-0 wild-type and *atg5/atg7* plants were transformed using GV3101 strain of Agrobacterium tumefaciens carrying pMDC pHusion1 AtATG8a construct as described in (Dauphinee, A. N. et al., 2019).
+
+Col-0 WT and *atg5-1* plants expressing GFP-ATG8a line was described previously (Minina, E. A. et al.,2018), (Thomson et al., 2005).
+
+Col-0 WT plants co-expressing vacuolar marker spL-RFP and the autophagosomal marker GFP-ATG8a were obtained by crossing the corresponding marker lines previously published in Hunter et al. ( Hunter et al., 2007) and Thomson et al. (Thomson et al., 2005).
\ No newline at end of file
diff --git a/studies/PlantMaterial/resources/.gitkeep b/studies/PlantMaterial/resources/.gitkeep
new file mode 100644
index 0000000..e69de29
-- 
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