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+ ## Root length measurement for seedlings grown in RoPods and on standard Petri plates
+
+To compare the root growth phenotypes for seedlings grown in RoPods and in standard Petri plates, 4 ml of growth medium (0.5 × MS (*Duchefa*, ref. M0222); MES 10 mM (*Duchefa*, ref. M1503); 1% sucrose; pH 5.8; 0.8% Plant agar (*Duchefa*, ref. P1001) were pipetted into each sterile RoPod v24 and 40 ml were poured into 12cmx12cm plates. A strip of the medium was removed from the RoPods or plates using a sterile scalpel. Seeds of GFP-ATG8 expressing lines were sterilized using chlorin method (incubated in 10% Chlorin; 0.001% Tween-20 for 20 min then washed 6 times with sterile water) and then plated into RoPod v24, or in standard plate, either on the edge between the agar and plastic plate bottom or on top of the agar. Three RoPod v24 were placed into a 12 cm square empty plates. Both standard agar plate and plate loaded with RoPod24 were mounted on the automated imaging platform SPIRO (Ohlsson, J. A. et al., 2021) and imaged every hour for seven days under 16h illumination (Day imaging settings: ISO 50, shutter speed 65; Night imaging settings: ISO 250 Shutter speed 10). The root length was measured manually on obtained images using *Fiji* software (ImageJ 1.53t) (Schindelin, J. et al., 2012).
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