From c3cf47e5071709c2f4f4cb9bec72c0120fff4011 Mon Sep 17 00:00:00 2001 From: Dominik Brilhaus <brilhaus@nfdi4plants.org> Date: Mon, 21 Oct 2024 07:11:13 +0000 Subject: [PATCH] add figure4 to readme --- .../README.md | 8 +++++++- 1 file changed, 7 insertions(+), 1 deletion(-) diff --git a/assays/PlantGrowthAndImageAnalysisOfSucroseTreatmentAssay/README.md b/assays/PlantGrowthAndImageAnalysisOfSucroseTreatmentAssay/README.md index 09a95eb..1561c9f 100644 --- a/assays/PlantGrowthAndImageAnalysisOfSucroseTreatmentAssay/README.md +++ b/assays/PlantGrowthAndImageAnalysisOfSucroseTreatmentAssay/README.md @@ -6,4 +6,10 @@ Representative video of Col-0 WT seedlings grown in RoPod5 on a vertical microscope and treated with 0.5x MS (control – left panel) or 0.5x MS supplemented with 1 % sucrose (right panel). - \ No newline at end of file + + +## Figure 4 + +Monitoring sucrose effect on root hair elongation illustrates applicability of RoPod for long-term time-lapse imaging combined with treatments. Arabidopsis Col-0 WT seedlings were grown for one week in a RoPod5 using the described protocol. The growth of root hairs was firstly recorded for 4 h under control conditions. After that, the chambers were flooded with liquid 0.5 × MS (control treatment) or liquid 0.5 × MS supplemented with 1% of sucrose (sucrose treatment). (a) Representative images showing roots at 0 h and 8 h of treatment. Each panel is a z-projection covering 240 µm. (b,c) Result of root hair tip tracking for one representative root for control (b) and for sucrose (c) treatments. The final root hair length (d), the root hair growth rate (e) and the growth duration (f) were calculated as described in the Supplementary Methods. (d–f) The sample size used for each measurement is shown in the tables below the charts. A Dunn’s multiple comparison test, different letters designate significantly different groups, p < 0.01. The dot in the violin plots is the median of the distribution. + + \ No newline at end of file -- GitLab