diff --git a/assays/FluoresceinDiffusionAssay/README.md b/assays/FluoresceinDiffusionAssay/README.md index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..3d02fc506d5663353687cbeb638d0e05af86280b 100644 --- a/assays/FluoresceinDiffusionAssay/README.md +++ b/assays/FluoresceinDiffusionAssay/README.md @@ -0,0 +1,8 @@ + +# Fluorescein Diffusion Assay + +## Figure 3 + +Diffusion of chemical compounds in the RoPod. (a) Representative time lapse images of roots treated with fluorescein in the RoPod. Arabidopsis seedlings were grown in RoPods using the described protocol. Liquid MS medium containing 2 μg.ml-1 of fluorescein dye was added to the chambers immediately prior to the start of imaging. Maximum intensity projections of the fluorescent signal are shown on the top row, corresponding transmitted light images are shown in the bottom row. The circles indicate three types of regions of interest (ROI) analyzed for each root hair to produce the data presented in (b). Magenta circle, root hair tip; green circle, root hair base; white circle, background. (b) Quantification of the data illustrated in (a). Diffusion rate of fluorescein in the RoPod chamber demonstrates that chemical compounds reach root hair cells within 15 min of application. Data for two independent experiments is plotted for each type of analyzed ROI. (c) Dynamics of fluorescence accumulation at the bottom coverslip of RoPod. In this experiment liquid MS medium containing 140 μg.ml−1 fluorescein was added to RoPods containing only growth medium. The fluorescence was recorded in four separate chambers, with 2 to 3 fields of view chosen at random positions in each RoPod. + + \ No newline at end of file