diff --git a/studies/RoPodChamberPrinting/README.md b/studies/RoPodChamberPrinting/README.md new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/studies/RoPodChamberPrinting/isa.study.xlsx b/studies/RoPodChamberPrinting/isa.study.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..6b514b3f66899049f63e0568d7c9cb5d004e6dbd Binary files /dev/null and b/studies/RoPodChamberPrinting/isa.study.xlsx differ diff --git a/studies/RoPodChamberPrinting/protocols/.gitkeep b/studies/RoPodChamberPrinting/protocols/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/studies/RoPodChamberPrinting/protocols/RoPod-Chamber-Printing.md b/studies/RoPodChamberPrinting/protocols/RoPod-Chamber-Printing.md new file mode 100644 index 0000000000000000000000000000000000000000..1eeacea3b57411bfc56ebeaab66a8818d12c7dc2 --- /dev/null +++ b/studies/RoPodChamberPrinting/protocols/RoPod-Chamber-Printing.md @@ -0,0 +1,7 @@ +## RoPod Chamber Printing + +In this study we firstly tested five types of microscopy coverslip bottom chambers from three different providers: Ibidi chambered coverslips (cat # 80,421 and 80,286 #1.5 polymer coverslip, hydrophobic, sterilized, Ibidi, Germany), 1-well II Chamber Slide™ System (Nunc™ Lab-Tek™, cat # C6307, Sigma-Aldrich), x-well cell culture chamber, 1-well, on coverglass (cat # 94.6190.102, Sarstedt). Plants in these chambers were grown using the same protocol as was later implemented for the custom designed chambers (Supplementary Methods). + +The different functional designs of RoPod chambers, as well as an illustrated step-by-step protocol for their 3D printing are presented in Supplementary File S1 and Supplementary Methods. Updates of the designs and of the printing protocols depending on the model of 3D printer used are available in the dedicated GitHub repository: https://github.com/AlyonaMinina/RoPod.Hardware. + +Printing of the chambers was tested using Prusa MK2.5S, MK3S, MK3S+ , MINI+ and Ultimaker S5 3D printers. We recommend using clear PETG filaments provided by Prusament, Sunlu or Prima Select \ No newline at end of file diff --git a/studies/RoPodChamberPrinting/resources/.gitkeep b/studies/RoPodChamberPrinting/resources/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391