From e8e4aa576e21bfbb0cbdccf459b7aed0940729c3 Mon Sep 17 00:00:00 2001 From: Dominik Brilhaus <brilhaus@nfdi4plants.org> Date: Mon, 21 Oct 2024 07:12:46 +0000 Subject: [PATCH] add partial fig1 to readme --- assays/RootLengthMeasurement/README.md | 10 +++++++++- 1 file changed, 9 insertions(+), 1 deletion(-) diff --git a/assays/RootLengthMeasurement/README.md b/assays/RootLengthMeasurement/README.md index deb7997..a759e86 100644 --- a/assays/RootLengthMeasurement/README.md +++ b/assays/RootLengthMeasurement/README.md @@ -10,4 +10,12 @@ corroborates that RoPod provides favourable growth conditions for Arabidopsis pl were acquired using SPIRO. - \ No newline at end of file + + +## Figure 1g-i + +RoPod, a toolkit comprising plant growth protocol and microscopy chamber optimized for long-term time-lapse imaging of Arabidopsis roots. + +(g–i) Growth conditions in RoPod chambers are equivalent to standard growing conditions on Petri plates, as demonstrated by comparing root growth on the top of the medium, under the medium inside the standard Petri plate and in RoPod chambers. Seeds of wild-type (WT) and autophagy-deficient atg5-1 Arabidopsis plants expressing GFP-ATG8 were sown on the agar medium in Petri plates or RoPods. Plates and chambers were placed on SPIRO under long day conditions and imaged for a week. (h) Zoomed-in insets shown in (g). (i) The chart represents data from two independent experiments. The root lengths were measured on the 7th day after seed plating. One-Way ANOVA test revealed no difference in root length of seedlings for the WT seedlings grown in RoPods and on Petri plates, significance level of 0.05, n = 103. + + -- GitLab