diff --git a/assays/GUSHistochemicalStaining/README.md b/assays/GUSHistochemicalStaining/README.md new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/GUSHistochemicalStaining/dataset/.gitkeep b/assays/GUSHistochemicalStaining/dataset/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/GUSHistochemicalStaining/isa.assay.xlsx b/assays/GUSHistochemicalStaining/isa.assay.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..73af5acbc35a29031af65e1b0e8a21d5363cc546 Binary files /dev/null and b/assays/GUSHistochemicalStaining/isa.assay.xlsx differ diff --git a/assays/GUSHistochemicalStaining/protocols/.gitkeep b/assays/GUSHistochemicalStaining/protocols/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/GUSHistochemicalStaining/protocols/GUSHistochemicalStainingProtocol.md b/assays/GUSHistochemicalStaining/protocols/GUSHistochemicalStainingProtocol.md new file mode 100644 index 0000000000000000000000000000000000000000..e41f48d05196e728047d4375e4ca2e29da850da9 --- /dev/null +++ b/assays/GUSHistochemicalStaining/protocols/GUSHistochemicalStainingProtocol.md @@ -0,0 +1,3 @@ +## GUS histochemical staining + +Three-week-old plants were carefully removed from ArtSoil and rinsed with 70% ethanol. Each plant was inserted into one well in a 12-well plate before 1 mL of GUS staining solution (10 mM EDTA, 50 mM phosphate buffer, 0.1% Triton X-100, 1 mM potassium ferrocyanide, 1 mM potassium ferricyanide, 2 mM X-Gluc, 20% methanol) was added into each well. The plate was subsequently incubated in the dark at 37 °C for 1 h (SWEET1, 2, 17) or 4 h (SWEET4, 7, 8, 11, 12, 13). Prior to imaging, plants were removed from GUS staining buffer and rinsed with 70% ethanol. \ No newline at end of file