From 30e364dfd2400885774d11403caf0f62bca5ffad Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Sat, 9 Nov 2024 16:18:25 +0100
Subject: [PATCH] add GUS histochemical staining assay

---
 assays/GUSHistochemicalStaining/README.md        |   0
 assays/GUSHistochemicalStaining/dataset/.gitkeep |   0
 assays/GUSHistochemicalStaining/isa.assay.xlsx   | Bin 0 -> 6905 bytes
 .../GUSHistochemicalStaining/protocols/.gitkeep  |   0
 .../GUSHistochemicalStainingProtocol.md          |   3 +++
 5 files changed, 3 insertions(+)
 create mode 100644 assays/GUSHistochemicalStaining/README.md
 create mode 100644 assays/GUSHistochemicalStaining/dataset/.gitkeep
 create mode 100644 assays/GUSHistochemicalStaining/isa.assay.xlsx
 create mode 100644 assays/GUSHistochemicalStaining/protocols/.gitkeep
 create mode 100644 assays/GUSHistochemicalStaining/protocols/GUSHistochemicalStainingProtocol.md

diff --git a/assays/GUSHistochemicalStaining/README.md b/assays/GUSHistochemicalStaining/README.md
new file mode 100644
index 0000000..e69de29
diff --git a/assays/GUSHistochemicalStaining/dataset/.gitkeep b/assays/GUSHistochemicalStaining/dataset/.gitkeep
new file mode 100644
index 0000000..e69de29
diff --git a/assays/GUSHistochemicalStaining/isa.assay.xlsx b/assays/GUSHistochemicalStaining/isa.assay.xlsx
new file mode 100644
index 0000000000000000000000000000000000000000..73af5acbc35a29031af65e1b0e8a21d5363cc546
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diff --git a/assays/GUSHistochemicalStaining/protocols/.gitkeep b/assays/GUSHistochemicalStaining/protocols/.gitkeep
new file mode 100644
index 0000000..e69de29
diff --git a/assays/GUSHistochemicalStaining/protocols/GUSHistochemicalStainingProtocol.md b/assays/GUSHistochemicalStaining/protocols/GUSHistochemicalStainingProtocol.md
new file mode 100644
index 0000000..e41f48d
--- /dev/null
+++ b/assays/GUSHistochemicalStaining/protocols/GUSHistochemicalStainingProtocol.md
@@ -0,0 +1,3 @@
+## GUS histochemical staining
+
+Three-week-old plants were carefully removed from ArtSoil and rinsed with 70% ethanol. Each plant was inserted into one well in a 12-well plate before 1 mL of GUS staining solution (10 mM EDTA, 50 mM phosphate buffer, 0.1% Triton X-100, 1 mM potassium ferrocyanide, 1 mM potassium ferricyanide, 2 mM X-Gluc, 20% methanol) was added into each well. The plate was subsequently incubated in the dark at 37 °C for 1 h (SWEET1, 2, 17) or 4 h (SWEET4, 7, 8, 11, 12, 13). Prior to imaging, plants were removed from GUS staining buffer and rinsed with 70% ethanol.
\ No newline at end of file
-- 
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