From 30e364dfd2400885774d11403caf0f62bca5ffad Mon Sep 17 00:00:00 2001 From: Viktoria Petrova <vipet103@hhu.de> Date: Sat, 9 Nov 2024 16:18:25 +0100 Subject: [PATCH] add GUS histochemical staining assay --- assays/GUSHistochemicalStaining/README.md | 0 assays/GUSHistochemicalStaining/dataset/.gitkeep | 0 assays/GUSHistochemicalStaining/isa.assay.xlsx | Bin 0 -> 6905 bytes .../GUSHistochemicalStaining/protocols/.gitkeep | 0 .../GUSHistochemicalStainingProtocol.md | 3 +++ 5 files changed, 3 insertions(+) create mode 100644 assays/GUSHistochemicalStaining/README.md create mode 100644 assays/GUSHistochemicalStaining/dataset/.gitkeep create mode 100644 assays/GUSHistochemicalStaining/isa.assay.xlsx create mode 100644 assays/GUSHistochemicalStaining/protocols/.gitkeep create mode 100644 assays/GUSHistochemicalStaining/protocols/GUSHistochemicalStainingProtocol.md diff --git a/assays/GUSHistochemicalStaining/README.md b/assays/GUSHistochemicalStaining/README.md new file mode 100644 index 0000000..e69de29 diff --git a/assays/GUSHistochemicalStaining/dataset/.gitkeep b/assays/GUSHistochemicalStaining/dataset/.gitkeep new file mode 100644 index 0000000..e69de29 diff --git a/assays/GUSHistochemicalStaining/isa.assay.xlsx b/assays/GUSHistochemicalStaining/isa.assay.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..73af5acbc35a29031af65e1b0e8a21d5363cc546 GIT binary patch literal 6905 zcmai3WmpvI5(cS7x>vfTmXeZ|P*}QKLYD4sknW{Kx};OO5ecO`6i_6jk&*_ziyYMB zIrrZ02M^CO?>F<jGdnZii=qqy;%zuMH~?I;ySY~EY&)tJJRICEA{-nZ>{A_aTN@{c zjg!8ryB)+)kJZiEsw_dyrh^?j=vYW{83abxQ&p4f$f@ZkEcyyJ?k2`UI=i3rYStB( zp_qxX*Z_|?nRmxCqw9`BU6k|`P)Z;*LWu_-b!LpwnyB$4?NvUMIvb$=)Sl3>QXDsa z(ME9IJvT5F^f@PbR76@D5{03-BB))i%F2a(%EOWy;(&^laNM~Nz>*$i_JmjmODVEI z^L1a>GNd1R{NNTbZtub4cU2DXA4&AYmYZ~S4nyL|0e4(oh>L3r;L!pFrOlXE;X~S7 zGh|0@$KMG&LB=Rqp6ZE0>t|a{d=H7oW7Jby$9=}Efl+U~jNqR`%)vvyIaLa8jdFP* zFI%PakQdY8>rOAusrS-15AVRCd=x2r(-<3}qoEq8uDIwS4gy7FD$2>W_d>j$zVzpB z=6)C|L)Hm-)t?0Sa4=53>wQamT_<p86-c$w|3V1&{n<vPkpOeh2BMCONa-Sp;$NA7 z`;iG4`9BXb>^FS}h?OJTjm>qo_A{kt)?t5>xdjJ@_S1&-%Cao(wR|T#y6BFV<nnon zjT9g|ubDFU13i~;JFN{#ZPbgdfG;~?F{0>E<|xtjTdSu1n}dQ;8&S$fr06Uq#P~@4 zLm@-${ol;(v6#@1LugPq?Q+pmS4=KtX~0FjtxzojsUBpH*pn>hIw83v=4kvrg?@7} z#^H(eP?z@Tuf^p%PG`M-D}hCIL!vWHbcL53W879YI=1Vv+lD!mrrH;M492o#VNcyB zcG7}1R<fBCM^^RJr<*T?3NoZdva{;a%y3kS<QMz3UpbS`>Y=U<N6LuRe{UpB3G%0L z)k1x8!8vx9cf<?shLL}=h5%#4&FUv*|1?0oHuyJY18FPOub6FwF-!crnX9dXrID?z z<<&RghG>gUc5I2A%iER5+Ai8)##l$jr@A`F2!S<qvR2Y&yw;b;Y9vymfNYe^uF`kO zZ>2T|kNH9sWG7H6vY5cwYjQ7JcksQqX3tHryJmER5TUo}Y2Qt_Zt{|Ht&%x-)5gjP zGTII7NrE{sAIV9Et{upcj1}JRpXe<deC*1g0Xd}|pF`L{Q>d)IJvO9HnZmf}+af-D zgsdNMKXUC997vlHJitbr&TowLNY9ueW_n3O=8faWZpX7sLCg{zbgT&VPflXzLyRK3 zEn%l8gab=)?D|yOy;S>mmbuO(2j3Rmao=0op!n}ZV_p+<MUA5w1mg5(nbwCZH3h<m z?0^vt{D<ui2*(wl&k~hvQ`xco=8u_U=1KT?X{l}%yC~I4r*c`RA8i5gN^d=^i?2IB zZ03_$F3+08isd5_#yoFJJ4jtEkHQoqDLV6SXQ7H+Yb-W?#oRL-a$Z6Z776k0)}D;; zZvlwg^kJRGk3sWsIvxVn<Q@=PhB%Xwd<>0FP9~Os-m4uWs|If9Ss{ZoKa+3FB+>U$ ze@WWv4SPAYV_4XfsB48k@0wG8H<S=moz!N&Y%o+4{R(B8<FbLGykP@b!V1E?9T5;$ zxU@Ok{MfwCdapXuaOfm?+n@JD-k~hAcpKf`7ti~AP%Eulis=BnNJOe3G?v5K11jBN zh-n!3FnG+`Ys+6OF?dvVS)L(`+Gxr0MF~t@TVJ--O=OP^k|&^b7dld^wHbgkl|Sa` zu+y#Z&t3maKw_emY<Y;k(1-A4zdiaKU<q_*O&-OMUwP|kyN68538d(wg<?U-9WSUh zI$lr-ZtNMPxsr7>AGtZD@nS6u3Vl4}W5Za;BexYE(h1xpBxfUA1&O=KK<k04Wnym3 z(0bBU2s!i{^tpS>WYFr$my1TL3%wXb0?$Q_Rf$INB#|~zcBMV+T<jJHvz#@m9_n3g z|FEVI%Gh>ZRC+LlzwRx4`lLc1z!<7mrhyn7fb+Ijo<RpZAnvwTi2?W10sojbL3Tqd zjH-~cqmd#L9Am7*?d<O}c%H4+1_FD}*3-A-Cc<Z)+R=P1f;L%}AiD;s)u>Gse<9_5 zR~IDwu<$`yf)L7H*tz(-OKe6E#{<_hhMugNNm*?#;9FnjG&A0SE)(%;_d3ln^hURd zsyL`DkQD!|$X(q^?HQCq8TC5*^!Gu}k9-Fx+elyaiQH8Ab%WjhFtut58;1h0afp9i zxsGOr4iIA%CkJyIQ^y}w8$Sr^wAg_=f<d1J?API`5^}>85u|EVr9D42(9XVz^Yx#8 zusyq^3V@HwIa>Q*yQQ_wm$6-Il({CQ9lx7u)^R7LZ)$7{w@qtx+BGc}MJ1pL8P7*_ zvHB|${XUBn23^ZuTPu-&F*3KJu?|;ub*+2=)}wm73I4zoVu^}pN%HjdVgX!fYIXO7 z7seS)(_<;fgfH;Nl8lMZxtRO6K!n0klKLmCxUZjrunQdvN8Rd#Yuw1Yr`EXoi*FZE zq<{o2qrc!<n+s@AF|VAyw1`rBQd-sA8CvE$LRXHQN0g+^xO?dl{92dESZ3sITefYy z+gyS|T+8+}h*>cYs|4L1CytVzbM*1(x8;2ws1-Nc{<IMXTi?Q44XQB1Ga|qq@&0Z| zU!DMpPEU#u$vPbaG14kkf<=NR_X1v|r~i1b?{RgLi(&WDfD7|8L<$G?J_7ZH6S+r= zac1co!47mCI|{G<N1y+zjXRk^tRX+U{dNB0hPdxW!lq{z<@GuP8wb~BKYspbI@?+b zwzE9=-<kZslpE{pXJ*+L_PdPpnESuk9-*NbCwv0tl41%|!UxWbvmzinb+RJ3%o+mK z4Up8gs<7~~x~IHDg`~gH%&4Z;nU5OU!=>i>?CiZgCa2KvDw$Rgw;+AO3W(4)ISsca z=G%WCBe9g|Zac!pEh{dCkAVZhu}QwiGO#&ao&&n`37dqHEDSWkiNIK(lSt<Ue7JZ! zbrFye1;mOCWTns%kCqp;lqw6io-W0X?ct)v_aAE=4Lx}Wq-7e++b0jIkP0_$FSuw| zFddM|eYcRCb`hGq%fK6rW1Y-C9FvZ|Tf~JC9vFxM8kZNzcit|p#m}h{<+(epAmZ5A z*lwpR{rTj?j&Y5zT*Z>@P=oU_wc&BA#^z+=nKInIx2Cz)i|WUFY4>?h`cjBLeDJ8( zR^PpUIf>ylEB6iG%Q?uEXZNzLzRD0Yf%^kS%dELeQ?ZY0P=)oUO_%1)2?DR%w;96d zNz7);)LOeOp2y(4nALV0cRoApBW)AYYFz7ErqRN;q~bSf!Sb7El#1(#sb(anqOc>+ zS&qSb5)z33XkiRb@-u8UI^JgTy6Y=H0~QLeWABqT6>ob71fKvqR*ji-RzM>bE0k>d zEqtCA7h8KfrV1HpLf@Q)kMrkv!r!<zPK$US3}fjN`+h%K+ANsg*rSH}9`<Wgd^<Ui z+sG5!Y&NaP5b->{oc6uEP(`qHbV>@rk_rv7N67@bY5~wDWc{#Co2AijN#LX6!zm+d z^@_rOOr|RCbbe{kaZbt^gy}{jtlU73D3~aL&_Wh?+wM8XjG=CG+H9}x>F2IdO_BT1 zgN9}R+2+d86zN<<0rtK`!5G?0F~>~*`cdE`P2%2rJg+rrj{LB@%bUAaWnac}kz>CI z2%Vxua9}tTo25m3HQ<E!dNSPFWru|9q4P%biKb*nov3O7u1a?#uOf?5AtX{P<<S?) zJ8M$p0}T~GU8&u#Koyg(!J2^d0rE^nCtLC0(Pr=GLt+HIbjfQm0-=?HRsHmj{W^*g z?}hmb9_1&AhEhMtlm?;Ze~HP(@)1?aZr;ITnYY%N4?xewidmK(aMrnX&sk(>4PcnQ z&x7@BYi31)ge={yH1G)X4SsqS)tfi1sr-VeoI>k5@W#)qOREP#xo<4SpfCM68H+5r zqUpp?-y;Z`AbGi%T85bh=V0$q54zG@^nCr4FrGgqw4soo3U;e9Kcqd92p!6E77+z& z(s^e?r|&>NIzyihjC%Hba!q{N<zq98j@^CccZZUJ-D#ar!l+fMDqXo!lbKhUce@W` zO1l*=Zmq0)So{5wPfFG53j#qR{SExQgu`0}fMW_4%O-~CBaSrM=W2rxwFBQ$i}0g; z2*^>ZUw4W#voulb8JqeykU}vXi`VCS)CuH0ce(|rL0*VkmxdI`<MK>3UY%SRS@$#L zUq{^s&mx=dqfrM0z{we=IPiCB4yZwcI3TyccdHd$wLQ8QSV>G?eBNKoqX$qn5{Hag za$p9f3_P?voSbdWJFDR(biZX&{Plv?A-;&Vm>BnP<5VhPJHlvm;-y)_d{;-GI}+wy z1UlPJy}M5k7m%rhWG@nW*;+goGFUnDo3_GD+~rm~1j}N2f>OcMM8WW+<eQyDTbm{- zI~(DN4_o&*ysWKPkco?gmJpA0Cy}^?Q|bnAF2)fD?QWk*#1&zaJpzqCcNBl6V3O=W z)hva0hs8UgOoSHKF(3}xNN5L{xUAtBv-9}@-E@tJNWa#m{F@gzN08S&#c0EvbTb7M z0C|2E<S~@jyIp*_9IqnU6>VD6fOb-Wy4sFXZmbO&>bf!-2W5r2b)|I-Ipc7H3JG4E z!=KDOrSH~9C#;_pv!tV9y}gX!bDn3dN#sJqjPWoeJa-H6idIt_?wed`GU@a=VBA|k z5sTIqS12nkP<L|CHFyw{TCQ$T56<Ql+2zuNYBOtS<hMrYSi86(#?A*>aOW!<v%Qti zzBnR0j@6=<dPMa8vnnyiUC=@Hb{$#8q6b~`!Vy@0vQh@4UJq2OSFio05z;(cBzWsZ z4<$?U`#J9u)6Aij=SS-&Z@Rr37-}m{SZXx2QFMyzJnZ$$@%86O5Lpghs;~2w49b^H zl-8Zo70j9^q&zND*_dkasGmQadszLwhbvm;<#@S=eAzU^WM77cgN2LOt5KH8ba3AG z#B5Z?xDQu7cd6H2GrMiy=FF?VFCRz(eve&XYcm3DfMNW#d^ovVL4Ir<c?o*3T^T=k z=a`vgUIhVzCCtylJY2OXm5DpvWz$c(^bFz7c&yy{jsP+)uJcU(QQzANXy(D$y~g!i zQMLgt-4c8h4dI8f1Wn5p&lf9F=Te_cXcmK+YK$0#Un0*NQ^dU?NA#SzXM%&R8o$_D zdl(m!|L&f2Dzn}_B${VDaoTGsWabu~X73xMm19PZpHMSuxKXk|_C&Cy2OV`hgA%!G zz50>rLo$M5SrU&0@-RN!wwpn^r=7(bD(6-@B2y7A^%(eJt?w~>!dmenlIOPb1HwQ; zJk*!AG)OpmorViq%H|Lf)irgkcive7A^oO#uS(`H?%VhD#fhO=#{?-4^ddSjHS%Q< zF)5SsXY+O-KX7;#{`i6e@Id6z=#vf*ftO_iUaw-dE%7G@D*19TL4ejR5}RbD`%WW3 zTTJdGB|C0p+00|;R(f^_W#kb3SB!}d7AhTTX-7*8kT8R1L8!qyN9DCm^fn{f@77ot z?|(3t3=$_djf6Kqqwk7uu9z=IbJ3uh?9-h&=j^uI`>+9}0upz^-*Y1Up2qWXK1x#0 zxmP6nT#V>zeE6Jn{z-%<iIG^Ab$`(34Xog&S}yU^J`+uE9kh^ubR}lYTPs{ii;Uj} zz#sc|6ApXyYsjkePhO|1VmA|)R%1nN>r4=5EM~fvKqC(I$UPi=xsOezS@sD>Ew&}A zXD2<<*)B$2T{<6G%<q}J4MC29&`|bAMvx7P$Z3ZU^9Nk|d~ZK$JeCBWXXdmY?^}xO znsB6h9-Q#+?=t|V_pF?WK943JuSLBIRPZ<8qyT}Q%i49bZrcv2@zJh*gWxQ?;ToT{ zRnOlP=)b?EjcuPOI@sF%`TXns+zAPUEz$I_2RSyZj(?gN+Sy$#(CKjxY-icggLcjd zg1<zi*)Rl)s&nL$cxYC_NzX2|_d2ntCL+di$6cQFVlMJ;$~OzeVGU<87SD>WhLhvH z|HNFWn~)=FD`tJ!h6`=B)yR~5fZm2oB&fa^N^5h7QY%emWE7#gjHvL;EO`kG<^;Kj zMhG6cj|EP^uX1if%!n;_S(BzQh59$ET4LP{zH(1#*x$x4=u#X0PNI!+Z+CS33(zq` zUEj!Q3T`!X0&6N)G<#uj^L3VrgRts-vxpULE#VF2UiYFpWYTiSmp1J<Os7+d$AHcu z9s@k{$%EoYom2$IzN=+=5sO6Fn0(LHsI+!j<m*&8EQrG5eK6O9v?b{23RW0aKQ`87 zes$n7wl`0}lbU)SD$tL){;2wC*LTXtLt&4QIiA!YiaZ5G?_dv8oHP57H35Wo6y-PI z1-I;X<u{rS*}ei@7chatGHq7H)@Z1k7p&tnvKY>p=J6yKn}iqSo7JnA27F-!<L@^= z59VBGCN9yXd4k*Y8onXiB=&l34x`lP)Q4qk3D$jZe$UtwTL;LGlqvULy?I&=2x51N z;)Ss#%$eXf!oX7aPaE!d&Zf}UL2RWDj`^VZQEOqdLql6`6!JuGsj+<8`HLpgx?Y4L zW;GBhTdyu#x1#SzeTrdx?NDYQ&tzvD`yqFZR#(TZa>h>*m38-FAKlzQFXkw10)Jb8 ze`Bl$gMv*B2<Tx}Kf&H+s*|<E(3FQniHClt3`2ZeAZ|!`_v09;)kh#eo+{)PK0h(k z$YYB7U56gXEC~%lRmo|m=0WXwINJMIGx2j_8kh3CudImraK&I@w(4OqUknR@-~u7g zBDJ-sbu16OE?K^!WQ3^||3!?Vv2&W&&T3p}w-WAc8Q0p*L|j0i$XoJaWt{~<0nx)e zV2Ao_ao$i*K-zuXQ%76c(2$L}O?{?qm)RjW>Rkcp8H|ji<KCrl59um<u8d%N(uc>J zZi!v-#ypp$p5zmm7!hA$eFZYmn@*9xbDkspJsTz<aUWe+Hk@GDz`C9TzpGGDadHYU zr7w8rBAns43={xAIU@~-ad|-M$GorX8j;A>y{`FP$mUTjHW91+Ru@!XK&wn)DwO8o z*hjUhq*diCm9|kEjo$j{g7;<k3GLhlEuG*aDF;3MQekgFt5y-fkgIC1H#mEMQ`xgd z)RAIjPkQr3P?yad1fN<=+_TGA&Lw%Zf56{mUzTCX7uaT9+ksNSAHkYVg@Zw>uHZ># zmZNn@krw2()*<Uo0<4^TSl|-~HdJeTf1kq8vSCxjUMSZ69L2geJi{w>CGw2$v|yfk zIDB!=q1B@H_3^<3Qs6GSOQBINyXbo5C~7L$L2gN8PlM8Bp=~@ky;-s?M(gqbmraT5 zotWtub4kkDMSg!t3q$UwIMgMXiARb;At7^0WZpU)<*6oocs8oJsKS1Lvpqc8Xi{!- za#S=%?)k{n6$>~Bp7c|tQe$?S_52<-+czZfLuY7;GVr(X;r_ck4$H=G=U>F~Ul;td zWnQVe*XOD$hQl&^qeTAQ>7Od^4GIpf{3qsz6#2iLU5TOB=gL_-tdxF8qc@)Z8Qs;Z z`ubb}7OnyR9^jv1=?xI}PX(A;n4fFd??wB6<Gs=pug{fxMwtEryXS8T<4x$-E$dH> z@dgbCr}hu*FU9c}WB;!A{JI4T<wpenP=9{i@c$?*1pK(+MyB|C_<sr&zZ?($4*Mlr z{PKGp`Jc<;uL~k!Jp;y_<ImOc7xe#y48r|wTK|Gwd;D{(|MIB%JM6C^|0nv-5%UX; t3=`UZt_U{+=TG>bJ@FSjf#7GU-01X*GDttZYuK>$;|;7L$%wAZ{{wC7a18(e literal 0 HcmV?d00001 diff --git a/assays/GUSHistochemicalStaining/protocols/.gitkeep b/assays/GUSHistochemicalStaining/protocols/.gitkeep new file mode 100644 index 0000000..e69de29 diff --git a/assays/GUSHistochemicalStaining/protocols/GUSHistochemicalStainingProtocol.md b/assays/GUSHistochemicalStaining/protocols/GUSHistochemicalStainingProtocol.md new file mode 100644 index 0000000..e41f48d --- /dev/null +++ b/assays/GUSHistochemicalStaining/protocols/GUSHistochemicalStainingProtocol.md @@ -0,0 +1,3 @@ +## GUS histochemical staining + +Three-week-old plants were carefully removed from ArtSoil and rinsed with 70% ethanol. Each plant was inserted into one well in a 12-well plate before 1 mL of GUS staining solution (10 mM EDTA, 50 mM phosphate buffer, 0.1% Triton X-100, 1 mM potassium ferrocyanide, 1 mM potassium ferricyanide, 2 mM X-Gluc, 20% methanol) was added into each well. The plate was subsequently incubated in the dark at 37 °C for 1 h (SWEET1, 2, 17) or 4 h (SWEET4, 7, 8, 11, 12, 13). Prior to imaging, plants were removed from GUS staining buffer and rinsed with 70% ethanol. \ No newline at end of file -- GitLab