diff --git a/studies/ArtSoilPreparation/README.md b/studies/ArtSoilPreparation/README.md new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/studies/ArtSoilPreparation/isa.study.xlsx b/studies/ArtSoilPreparation/isa.study.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..151dab8fb2be1d0a8e7b6846854bba24f2241cb6 Binary files /dev/null and b/studies/ArtSoilPreparation/isa.study.xlsx differ diff --git a/studies/ArtSoilPreparation/protocols/.gitkeep b/studies/ArtSoilPreparation/protocols/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/studies/ArtSoilPreparation/protocols/ArtSoilPreparationProtocol.md b/studies/ArtSoilPreparation/protocols/ArtSoilPreparationProtocol.md new file mode 100644 index 0000000000000000000000000000000000000000..388d10d19bbba169f794f2cea1fb726dad6c668b --- /dev/null +++ b/studies/ArtSoilPreparation/protocols/ArtSoilPreparationProtocol.md @@ -0,0 +1,3 @@ +## ArtSoil preparation + +For the preparation of 10% autoclaved soil suspension, sieved CAS (to remove pebbles) was dissolved in deionized water and autoclaved three times before use. Live bacterial suspension was prepared by inoculating fresh CAS into 10% autoclaved soil suspension to achieve desired bacterial dilution (Korenblum, E. et al., 2020). Autoclaved half-strength Murashige-Skoog (½ MS) media including MES (Duchefa Biochemie) and supplemented with 1% agar was cooled to 40–45°C before it was inoculated with live bacterial suspension. Approximately 50 mL of ArtSoil media was used for each 12 cm x 12 cm culture plate (Figure S2B). Sterilized *A. thaliana Col-0* seeds were sown and germinated on ArtSoil, and incubated in a plant growth chamber for 4 weeks under standard growth conditions. \ No newline at end of file diff --git a/studies/ArtSoilPreparation/resources/.gitkeep b/studies/ArtSoilPreparation/resources/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391