From 4820b8a8b0f316b2715296ba577effbabcf9ba74 Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Sun, 3 Nov 2024 12:57:38 +0100
Subject: [PATCH] add art soil preparation study and protocol

---
 studies/ArtSoilPreparation/README.md             |   0
 studies/ArtSoilPreparation/isa.study.xlsx        | Bin 0 -> 7499 bytes
 studies/ArtSoilPreparation/protocols/.gitkeep    |   0
 .../protocols/ArtSoilPreparationProtocol.md      |   3 +++
 studies/ArtSoilPreparation/resources/.gitkeep    |   0
 5 files changed, 3 insertions(+)
 create mode 100644 studies/ArtSoilPreparation/README.md
 create mode 100644 studies/ArtSoilPreparation/isa.study.xlsx
 create mode 100644 studies/ArtSoilPreparation/protocols/.gitkeep
 create mode 100644 studies/ArtSoilPreparation/protocols/ArtSoilPreparationProtocol.md
 create mode 100644 studies/ArtSoilPreparation/resources/.gitkeep

diff --git a/studies/ArtSoilPreparation/README.md b/studies/ArtSoilPreparation/README.md
new file mode 100644
index 0000000..e69de29
diff --git a/studies/ArtSoilPreparation/isa.study.xlsx b/studies/ArtSoilPreparation/isa.study.xlsx
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diff --git a/studies/ArtSoilPreparation/protocols/.gitkeep b/studies/ArtSoilPreparation/protocols/.gitkeep
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diff --git a/studies/ArtSoilPreparation/protocols/ArtSoilPreparationProtocol.md b/studies/ArtSoilPreparation/protocols/ArtSoilPreparationProtocol.md
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@@ -0,0 +1,3 @@
+## ArtSoil preparation
+
+For the preparation of 10% autoclaved soil suspension, sieved CAS (to remove pebbles) was dissolved in deionized water and autoclaved three times before use. Live bacterial suspension was prepared by inoculating fresh CAS into 10% autoclaved soil suspension to achieve desired bacterial dilution (Korenblum, E. et al., 2020). Autoclaved half-strength Murashige-Skoog (½ MS) media including MES (Duchefa Biochemie) and supplemented with 1% agar was cooled to 40–45°C before it was inoculated with live bacterial suspension. Approximately 50 mL of ArtSoil media was used for each 12 cm x 12 cm culture plate (Figure S2B). Sterilized *A. thaliana Col-0* seeds were sown and germinated on ArtSoil, and incubated in a plant growth chamber for 4 weeks under standard growth conditions.
\ No newline at end of file
diff --git a/studies/ArtSoilPreparation/resources/.gitkeep b/studies/ArtSoilPreparation/resources/.gitkeep
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-- 
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