diff --git a/.DS_Store b/.DS_Store new file mode 100644 index 0000000000000000000000000000000000000000..8a0f110c9d8a98c64496ec2e547360b3c50a5c42 Binary files /dev/null and b/.DS_Store differ diff --git a/README.md b/README.md index f934777e6cb0a128f9b1dd41a6ab90d132f5ede1..5a67b27808778581bb8883d97cdf0d02c5ca94ea 100644 --- a/README.md +++ b/README.md @@ -1,5 +1,11 @@ # Sugar transporters spatially organize microbiota colonization along the longitudinal root axis of *Arabidopsis* +## Summary + +Plant roots are functionally heterogeneous in cellular architecture, transcriptome profile, metabolic state, and microbial immunity. We hypothesized that axial differentiation may also impact spatial colonization by root microbiota along the root axis. We developed two growth systems, ArtSoil and CD-Rhizotron, to grow and then dissect Arabidopsis thaliana roots into three segments. We demonstrate that distinct endospheric and rhizosphere bacterial communities colonize the segments, supporting the hypothesis of microbiota differentiation along the axis. Root metabolite profiling of each segment reveals differential metabolite enrichment and specificity. Bioinformatic analyses and GUS histochemistry indicate microbe-induced accumulation of SWEET2, 4, and 12 sugar uniporters. Profiling of root segments from *sweet* mutants shows altered spatial metabolic profiles and reorganization of endospheric root microbiota. This work reveals the interdependency between root metabolites and microbial colonization and the contribution of SWEETs to spatial diversity and stability of microbial ecosystem. + +<img src="./_publication/1-s2.0-S193131282400057X-fx1.jpeg" style="width:100%;display: block;margin: auto; padding: 30px 0px;"> + ## Original Publication Loo et al., 2024, Cell Host & Microbe 32, 543–556 April 10, 2024 ª 2024 The Author(s). Published by Elsevier Inc. https://doi.org/10.1016/j.chom.2024.02.014