From 55631f74626feb3097273541ba06bd2c16f8261c Mon Sep 17 00:00:00 2001
From: Mekhrona Mirzoeva <memir101@hhu.de>
Date: Wed, 25 Sep 2024 11:39:53 +0200
Subject: [PATCH] Added Summary to README.md

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diff --git a/README.md b/README.md
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 # Sugar transporters spatially organize microbiota colonization along the longitudinal root axis of *Arabidopsis*
 
+## Summary 
+
+Plant roots are functionally heterogeneous in cellular architecture, transcriptome profile, metabolic state, and microbial immunity. We hypothesized that axial differentiation may also impact spatial colonization by root microbiota along the root axis. We developed two growth systems, ArtSoil and CD-Rhizotron, to grow and then dissect Arabidopsis thaliana roots into three segments. We demonstrate that distinct endospheric and rhizosphere bacterial communities colonize the segments, supporting the hypothesis of microbiota differentiation along the axis. Root metabolite profiling of each segment reveals differential metabolite enrichment and specificity. Bioinformatic analyses and GUS histochemistry indicate microbe-induced accumulation of SWEET2, 4, and 12 sugar uniporters. Profiling of root segments from *sweet* mutants shows altered spatial metabolic profiles and reorganization of endospheric root microbiota. This work reveals the interdependency between root metabolites and microbial colonization and the contribution of SWEETs to spatial diversity and stability of microbial ecosystem.
+
+<img src="./_publication/1-s2.0-S193131282400057X-fx1.jpeg" style="width:100%;display: block;margin: auto; padding: 30px 0px;">
+
 ## Original Publication
 
 Loo et al., 2024, Cell Host & Microbe 32, 543–556 April 10, 2024 ª 2024 The Author(s). Published by Elsevier Inc. https://doi.org/10.1016/j.chom.2024.02.014
-- 
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