diff --git a/assays/RootMetaboliteProfiling/isa.assay.xlsx b/assays/RootMetaboliteProfiling/isa.assay.xlsx index 123c5a7c2206f7a8e9a54523265ff0c499b76398..fe6ed3cc8511b91d33e1cc2b0657366ca3def604 100644 Binary files a/assays/RootMetaboliteProfiling/isa.assay.xlsx and b/assays/RootMetaboliteProfiling/isa.assay.xlsx differ diff --git a/studies/PlantMaterialAndGrowthConditions/README.md b/studies/PlantMaterialAndGrowthConditions/README.md new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/studies/PlantMaterialAndGrowthConditions/isa.study.xlsx b/studies/PlantMaterialAndGrowthConditions/isa.study.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..aff116b98ac05cebda89b5eebc4196be406e37fb Binary files /dev/null and b/studies/PlantMaterialAndGrowthConditions/isa.study.xlsx differ diff --git a/studies/PlantMaterialAndGrowthConditions/protocols/.gitkeep b/studies/PlantMaterialAndGrowthConditions/protocols/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/studies/PlantMaterialAndGrowthConditions/protocols/ExperimentalModel.md b/studies/PlantMaterialAndGrowthConditions/protocols/ExperimentalModel.md new file mode 100644 index 0000000000000000000000000000000000000000..e984a92a5e39ea926112a49cb24d1f6b95ae3618 --- /dev/null +++ b/studies/PlantMaterialAndGrowthConditions/protocols/ExperimentalModel.md @@ -0,0 +1,3 @@ +## Experimental Model + +*Arabidopsis thaliana* Col-0 seeds were sterilized in a 1.5-mL microcentrifuge tube with 1 mL of 30% chlorine + 0.1% Triton X-100 for 15-20 min at room temperature with agitation. The seeds were rinsed once with 1 mL of 80% ethanol, followed by five rinses with autoclaved ddH2O. The microcentrifuge tube was wrapped in aluminum foil and cold-treated at 4 °C for 2-5 days before sowing. *A. thaliana* Col-0 seeds were incubated under standard growth conditions, i.e., 22 °C, 10 h light/ 14 h dark, 60% humidity, 180-230 μmol/s/m2 light intensity. \ No newline at end of file diff --git a/studies/PlantMaterialAndGrowthConditions/resources/.gitkeep b/studies/PlantMaterialAndGrowthConditions/resources/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391