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 Here we used bioinformatics, proteomics and fluorescence imaging to identify proteins with similarities to phase separating FG-NUPs at PD. We identified 20 NUPs in PD fractions, and validated dual localization to NPC and PD for 7 NUPs. Structured illumination microscopy detected the transmembrane anchor NUP CPR5 at both orifices of PD. cpr5 mutants showed reduced intercellular transport of SHR from stele to endodermis. The identification of FG-NUPs at PD is consistent with the recruitment of NUPs in the green lineage to form a PD pore gating complex consistent with liquid phase separation domains as diffusion barriers at PD.
 
+![](_publication/biorxiv-preprint/Figure1.png)
+<figcaption>Figure 1. Identification of NUPs at plasmodesmata.</figcaption>
 
 ## Preprint
 
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@@ -10,4 +10,8 @@ See [2024.09.02.610746v2.full.pdf](biorxiv-preprint/2024.09.02.610746v2.full.pdf
 **Identification of nuclear pore proteins at plasmodesmata**
 
 T. Moritz Schladt, Manuel Miras, Jona Obinna Ejike, Mathieu Pottier, Lin Xi, Andrea Restrepo-Escobar, Masayoshi Nakamura, Niklas Pütz, Sebastian Hänsch, Chen Gao, Julia Engelhorn, Marcel Dickmanns, Gwendolyn V. Davis, Ahan Dalal, Sven Gombos, Ronja Lange, Rüdiger Simon, Waltraud X. Schulze, Wolf B. Frommer
-bioRxiv 2024.09.02.610746; doi: https://doi.org/10.1101/2024.09.02.610746
\ No newline at end of file
+bioRxiv 2024.09.02.610746; doi: https://doi.org/10.1101/2024.09.02.610746
+
+### Copyright
+
+The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a [CC-BY-NC-ND 4.0 International license](http://creativecommons.org/licenses/by-nc-nd/4.0/).
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