diff --git a/README.md b/README.md index acefdfbf239ec266f85b2cea80d61de7531a2dad..cd3113ecff4a4e5b9500a50dd3e970880166aa53 100644 --- a/README.md +++ b/README.md @@ -1,6 +1,87 @@ +# Identification of nuclear pore proteins at plasmodesmata + +## Abstract + Here we used bioinformatics, proteomics and fluorescence imaging to identify proteins with similarities to phase separating FG-NUPs at PD. We identified 20 NUPs in PD fractions, and validated dual localization to NPC and PD for 7 NUPs. Structured illumination microscopy detected the transmembrane anchor NUP CPR5 at both orifices of PD. cpr5 mutants showed reduced intercellular transport of SHR from stele to endodermis. The identification of FG-NUPs at PD is consistent with the recruitment of NUPs in the green lineage to form a PD pore gating complex consistent with liquid phase separation domains as diffusion barriers at PD. -Raw and meta data can be found in "assays": -NUP localization when transiently and stably expressed, topolgy of CPR5, structured illumination microscopy transiently expressing CPR5 and PDLP5, transport assays: bombardment, DropANdSee, short root SHR-GFP. + +<figcaption>Figure 1. Identification of NUPs at plasmodesmata.</figcaption> + +## Preprint + +A bioRxiv preprint of the associated publication is available at https://doi.org/10.1101/2024.09.02.610746 and [here](_publication/biorxiv-preprint/2024.09.02.610746v2.full.pdf) + + +## Raw data and metadata can be found in "assays" + +- NUP localization + - [transiently expressed](assays/transient_localization) + - [stably expressed](assays/stable_localization_in_reporter_line) +- [topology of CPR5](assays/topology) +- [structured illumination microscopy (SIM)](assays/SIM) transiently expressing CPR5 and PDLP5 +- transport assays + - [bombardment](assays/bombardment) + - [DropANdSee (DANS)](assays/DANS) + - [short root SHR-GFP](assays/SHR-GFP) +- [sequencing results for cpr5G420D](assays/sequencing_of_Arabidopsis_cpr5-1) + +## Contributors -Sequencing results for cpr5G420D can be found in \ No newline at end of file +- Till Moritz Schladt + - email: till.schladt@hhu.de + - Affiliation: Heinrich Heine Univeryity, Düsseldorf + - ORCID: [0000-0002-9160-4664](https://orcid.org/0000-0002-9160-4664) +- Miras Manuel + - Affiliation: CEBAS-CSIC, Heinrich-Heine-Universität Düsseldorf Mathematisch-Naturwissenschaftliche Fakultät, Insitute of Molecular Physiology Heinrich-Heine-Universität Düsseldorf Mathematisch-Naturwissenschaftliche Fakultät, Institute Molecular Physiology;Abiopep SL, ;Centro de EdafologÃa y BiologÃa Aplicada del Segura, Plant Virology;Centro de EdafologÃa y BiologÃa Aplicada del Segura, Plant Virology + - ORCID: [0000-0002-3636-0265](https://orcid.org/0000-0002-3636-0265) +- Jona Obinna Ejike + - Affiliation: Heinrich Heine Universität, Düsseldorf + - ORCID: [0000-0002-4601-8551](https://orcid.org/0000-0002-4601-8551) +- Mathieu Pottier + - Affiliation: Université de Lorraine, UMR 1136 Université de Lorraine/INRAE Interactions Arbres Microorganismes (IAM);Heinrich-Heine-Universität Düsseldorf, Institute for Molecular Physiology;University of Liège, Integrative Biological Sciences (InBioS);Université catholique de Louvain, Louvain Institute of Biomolecular Science and Technology (LIBST);CNRS Délégation Ile-de-France Sud, Institut des Sciences du Végétal (ISV) + - ORCID: [0000-0003-1551-4699](https://orcid.org/0000-0003-1551-4699) +- Lin Xi + - Affiliation: University of Hohenheim + - ORCID: [0000-0002-6811-9505](https://orcid.org/0000-0002-6811-9505) +- Andrea Restrepo + - Affiliation: Heinrich-Heine-Universität Düsseldorf, Institute for Molecular Physiology + - ORCID: [0000-0003-2870-394X](https://orcid.org/0000-0003-2870-394X) +- Masayoshi Nakamura + - Affiliation: Nagoya University, Institute of Transformative Bio-Molecules;Nagoya University, Institute of Transformative Bio-Molecules;Carnegie Institution for Science, Department of Plant Biology;Nara Institute of Science and Technology, Department of Biological Science + - ORCID: [0000-0002-7107-4942](https://orcid.org/0000-0002-7107-4942) +- Niklas Pütz + - Affiliation: Heinrich Heine Universität, Düsseldorf + - ORCID: [0009-0006-1153-5918](https://orcid.org/0009-0006-1153-5918) +- Sebastian Hänsch + - Affiliation: Heinrich Heine University Düsseldorf, Center for Advanced Imaging;Heinrich Heine University Düsseldorf, Center for Advanced Imaging + - ORCID: [0000-0002-7762-2516](https://orcid.org/0000-0002-7762-2516) +- Chen Gao + - Affiliation: University of Copenhagen, Department of Plant and Environmental Sciences + - ORCID: [0000-0003-4750-3900](https://orcid.org/0000-0003-4750-3900) +- Julia Engelhorn + - Affiliation: University of Montpellier, IRD;Heinrich Heine University Düsseldorf, Institute for Molecular Physiology;University of Warwick, School of Life Sciences;CEA Grenoble, Cell & Plant Physiology Laboratory + - ORCID: [0000-0002-6680-3012](https://orcid.org/0000-0002-6680-3012) +- Marcel Dickmanns + - Affiliation: Heinrich-Heine-Universität Düsseldorf, ;Max Planck Institute of Biochemistry, + - ORCID: [0000-0003-4001-707X](https://orcid.org/0000-0003-4001-707X) +- Gwendolyn Davis + - Affiliation: University of Warwick, Life Sciences;University of Cambridge, Plant Sciences + - ORCID: [0000-0003-4704-4863](https://orcid.org/0000-0003-4704-4863) +- Ahan Dalal + - Affiliation: Hebrew University of Jerusalem, Plant Science;Tel Aviv University, Plant Science + - ORCID: [0000-0003-1876-1484](https://orcid.org/0000-0003-1876-1484) +- Sven Gombos + - Affiliation: University of Hohenheim, Plant Systems Biology + - ORCID: [0000-0003-4177-526X](https://orcid.org/0000-0003-4177-526X) +- Ronja Lange + - Affiliation: Heinrich Heine Universität, Düsseldorf + - ORCID: [0009-0009-2475-2438](https://orcid.org/0009-0009-2475-2438) +- Rüdiger Simon + - Affiliation: Heinrich Heine University, Biology + - ORCID: [0000-0002-1317-7716](https://orcid.org/0000-0002-1317-7716) +- Waltraud Schulze + - Affiliation: University of Hohenheim + - ORCID: [0000-0001-9957-7245](https://orcid.org/0000-0001-9957-7245) +- Wolf B. Frommer + - Affiliation: Heinrich-Heine-Universitat Düsseldorf, Institute for Molecular Physiology;ITbM, ;Carnegie Institution for Science, Plant Biology;Stanford University, Biology;Carnegie Institution for Science, Plant Biology;Stanford University, Biology;Carnegie Institution for Science, Plant Biology;University of Tübingen, Biology + - ORCID: [0000-0001-6465-0115](https://orcid.org/0000-0001-6465-0115) \ No newline at end of file diff --git a/_publication/README.md b/_publication/README.md new file mode 100644 index 0000000000000000000000000000000000000000..be7989837a61d6a2304db53736635db8d1a8a7e6 --- /dev/null +++ b/_publication/README.md @@ -0,0 +1,17 @@ + +# Publication files + +## bioRxiv preprint + +See [2024.09.02.610746v2.full.pdf](biorxiv-preprint/2024.09.02.610746v2.full.pdf) + +### Citation + +**Identification of nuclear pore proteins at plasmodesmata** + +T. Moritz Schladt, Manuel Miras, Jona Obinna Ejike, Mathieu Pottier, Lin Xi, Andrea Restrepo-Escobar, Masayoshi Nakamura, Niklas Pütz, Sebastian Hänsch, Chen Gao, Julia Engelhorn, Marcel Dickmanns, Gwendolyn V. Davis, Ahan Dalal, Sven Gombos, Ronja Lange, Rüdiger Simon, Waltraud X. Schulze, Wolf B. Frommer +bioRxiv 2024.09.02.610746; doi: https://doi.org/10.1101/2024.09.02.610746 + +### Copyright + +The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. 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