From c73ba35ab8fb27012ed7f5bb8ab5c1a871e86ce8 Mon Sep 17 00:00:00 2001
From: Moritz Schladt <till.schladt@hhu.de>
Date: Wed, 27 Nov 2024 20:20:24 +0000
Subject: [PATCH] Add README.md

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+Here we used bioinformatics, proteomics and fluorescence imaging to identify proteins with similarities to phase separating FG-NUPs at PD. We identified 20 NUPs in PD fractions, and validated dual localization to NPC and PD for 7 NUPs. Structured illumination microscopy detected the transmembrane anchor NUP CPR5 at both orifices of PD. cpr5 mutants showed reduced intercellular transport of SHR from stele to endodermis. The identification of FG-NUPs at PD is consistent with the recruitment of NUPs in the green lineage to form a PD pore gating complex consistent with liquid phase separation domains as diffusion barriers at PD.
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+Raw and meta data can be found in "assays":
+NUP localization when transiently and stably expressed, topolgy of CPR5, structured illumination microscopy transiently expressing CPR5 and PDLP5, transport assays: bombardment, DropANdSee, short root SHR-GFP.
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+Sequencing results for cpr5G420D can be found in 
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