diff --git a/README.md b/README.md index cd3113ecff4a4e5b9500a50dd3e970880166aa53..a6c8eadfaa69749c34faf8d5efe686abb7704347 100644 --- a/README.md +++ b/README.md @@ -1,5 +1,8 @@ # Identification of nuclear pore proteins at plasmodesmata +[](https://doi.org/10.60534/46qra-6bw98) + + ## Abstract Here we used bioinformatics, proteomics and fluorescence imaging to identify proteins with similarities to phase separating FG-NUPs at PD. We identified 20 NUPs in PD fractions, and validated dual localization to NPC and PD for 7 NUPs. Structured illumination microscopy detected the transmembrane anchor NUP CPR5 at both orifices of PD. cpr5 mutants showed reduced intercellular transport of SHR from stele to endodermis. The identification of FG-NUPs at PD is consistent with the recruitment of NUPs in the green lineage to form a PD pore gating complex consistent with liquid phase separation domains as diffusion barriers at PD.