From f33bcf5d682b5f7aafa518b00f3d4499239999f1 Mon Sep 17 00:00:00 2001
From: Dominik Brilhaus <brilhaus@nfdi4plants.org>
Date: Tue, 3 Dec 2024 07:30:56 +0000
Subject: [PATCH] Update README.md

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 # Identification of nuclear pore proteins at plasmodesmata
 
+[![DOI](https://archive.nfdi4plants.org/badge/DOI/10.60534/46qra-6bw98.svg)](https://doi.org/10.60534/46qra-6bw98)
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 ## Abstract
 
 Here we used bioinformatics, proteomics and fluorescence imaging to identify proteins with similarities to phase separating FG-NUPs at PD. We identified 20 NUPs in PD fractions, and validated dual localization to NPC and PD for 7 NUPs. Structured illumination microscopy detected the transmembrane anchor NUP CPR5 at both orifices of PD. cpr5 mutants showed reduced intercellular transport of SHR from stele to endodermis. The identification of FG-NUPs at PD is consistent with the recruitment of NUPs in the green lineage to form a PD pore gating complex consistent with liquid phase separation domains as diffusion barriers at PD.
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