diff --git a/assays/qRT-PCR/isa.assay.xlsx b/assays/qRT-PCR/isa.assay.xlsx index b27673b9c40b008b0691c55e6a3df6d79b1732ad..329638d5dd3b39a1d2ebcfa56f2c517918fc6e22 100644 Binary files a/assays/qRT-PCR/isa.assay.xlsx and b/assays/qRT-PCR/isa.assay.xlsx differ diff --git a/studies/Fawke-2019/README.md b/studies/Fawke-2019/README.md new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/studies/Fawke-2019/isa.study.xlsx b/studies/Fawke-2019/isa.study.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..98f889102a53574a839857f092909761c31ae618 Binary files /dev/null and b/studies/Fawke-2019/isa.study.xlsx differ diff --git a/studies/Fawke-2019/protocols/.gitkeep b/studies/Fawke-2019/protocols/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/studies/Fawke-2019/protocols/qRT-PCR.md b/studies/Fawke-2019/protocols/qRT-PCR.md new file mode 100644 index 0000000000000000000000000000000000000000..9ca8775b5e69ad0f4705878e22d5b8e64aeb91ba --- /dev/null +++ b/studies/Fawke-2019/protocols/qRT-PCR.md @@ -0,0 +1,3 @@ +## Quantitative reverse transcription polymerase chain reaction (qRT-PCR) + +Total RNA was extracted from plant material using Qiagen RNeasy Plant Mini Kit (Qiagen), including 1% (v/v) β-mercaptoethanol in the extraction buffer. RNA was then reverse-transcribed to cDNA using the Roche Transcriptor First Strand cDNA Synthesis Kit. qPCR was performed in 384 well plates using a Roche LightCycler 480 SYBR Green I Master Mix in a Roche LightCycler 480 II machine. Three technical replicates were performed for each sample. Normalization of crossing point-PCR-cycle (Cp) values to an internal control was performed against NbEF1a, NbF-BOX or NbL23 (Liu et al., 2012) for quantification of *N. benthamiana* transcripts and against PiWS21 (Yan & Liou, 2006) for *P. infestans* transcripts. \ No newline at end of file diff --git a/studies/Fawke-2019/resources/.gitkeep b/studies/Fawke-2019/resources/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391