| Bacterial and virus strains |
| Escherichia coli MachI |
University of Cologne, Germany (Prof. Dr. Ute Hoecker) |
N/A |
| Agrobacterium tumefaciens GV3101::pMP90RK |
this paper |
N/A |
| Agrobacterium tumefaciens GV3101::pBIN61-p19 |
this paper |
N/A |
| Chemicals, peptides, and recombinant proteins |
| Horseradish peroxidase |
Sigma-Aldrich |
P6782; CAS: 9003-99-0 |
| L-012 |
Wako Chemicals |
120-04891; CAS: 143556-24-5 |
| Rifampicin |
Roth |
4163.2; CAS: 13292-46-1 |
| Kanamycin |
Duchefa |
K0126.0010; CAS: 25389-94-0 |
| Carbenicillin |
Roth |
6344.1; CAS: 4800-94-6 |
| Gentamicin |
Roth |
0233.2; CAS: 1405-41-0 |
| Sodium hypochlorite |
Roth |
9062.4; CAS: 7681-52-9 |
| T4 polynucleotide kinase |
New England BioLabs |
CAT#M0201S |
| T4 DNA ligase |
New England BioLabs |
CAT#M0202M |
| DpnI |
New England BioLabs |
CAT#R0176L |
| T4 DNA ligase buffer |
New England BioLabs |
CAT#B0202S |
| N-naphthol |
Sigma Aldrich |
N1000-10G; CAS: 90-15-3 |
| D-glucose |
Roth |
6887.2; CAS: 77938-63-7 |
| Laminaribiose β-1-3-(Glc)2 |
Megazyme |
O-LAM2; CAS: 34980-39-7 |
| Laminaritriose β-1-3-(Glc)3 |
Megazyme |
O-LAM3; CAS: 3256-04-0 |
| Laminaripentaose β-1-3-(Glc)5 |
Megazyme |
O-LAM5; CAS: 23743-55-7 |
| Gentiobiose β-1-6-(Glc)2 |
Megazyme |
O-GENT; CAS: 554-91-6 |
| endo-1,3-β-D-glucanase (barley BGLUII) |
Megazyme |
E-LAMHV; CAS: 9025-37-0 |
| Chitohexaose |
Megazyme |
O-CHI6; CAS: 38854-46-5 |
| Cellohexaose |
Megazyme |
O-CHE; CAS: 2478-35-5 |
| Xyloglucan oligomer (XXXG) |
Megazyme |
O-X3G4; CAS: 121591-98-8 |
| Laminarin from Eisenia bicyclis |
Biosynth |
YL02421; CAS: 9008-22-4 |
| Laminarin from Laminaria digitata |
Sigma Aldrich |
L9634-1G; CAS: 9008-22-4 |
| Elf18 |
The Sainsbury Laboratory, United Kingdom (Prof. Dr. Cyril Zipfel) |
N/A |
| EZ-Link Hydrazide-Biotin |
Thermo Scientific |
CAT#21339 |
| Sodium cyanoborohydride |
Sigma Aldrich |
42077-10G; CAS: 25895-60-7 |
| Aniline blue |
Sigma Aldrich |
415049-25G; CAS: 666787-07-8 |
| Concanavalin A (ConA) - Alexa Fluor 633 |
Invitrogen, Thermo Fisher Scientific |
CAT#C21402 |
| Wheat Germ Agglutinin (WGA) - Alexa Fluor 488 |
Invitrogen, Thermo Fisher Scientific |
CAT#W11261 |
| Avidin |
Sigma Aldrich |
A9275-5MG; CAS: 1405-69-2 |
| Acetosyringone |
Roth |
6003.2; CAS: 2478-38-8 |
| NeutrAvidin agarose resin |
Thermo Fisher Scientific |
CAT#29200 |
| Blue ink |
Pelikan |
301010 |
| Gelrite |
Duchefa |
G1101; CAS: 71010-52-1 |
| Strep-Tactin Macroprep |
IBA Lifesciences GmbH |
2-1505-010 |
| Critical commercial assays |
| NucleoSpin Gel and PCR Clean-up Kit |
Macherey-Nagel |
740609.50 |
| NucleoSpin Plasmid Kit |
Macherey-Nagel |
740588.50 |
| Deposited data |
| Proteomic data |
ProteomeXchange Consortium via PRIDE partner repository (Perez-Riverol et al.73) |
PXD046129 |
| Experimental models: Organisms/strains |
| Serendipita indica: DSM11827 |
German Collection of Microorganisms and Cell Cultures (DSMZ) |
NCBI Taxonomy ID: 1109443 |
| Serendipita vermifera: MAFF305830 |
NARO Research Center of Genetic Resources, Japan |
NCBI Taxonomy ID: 933852 |
| Bipolaris sorokiniana: ND90Pr |
Max Planck Institute for Terrestrial Microbiology, Marburg, Germany (Shadab Nizam) |
NCBI Taxonomy ID: 665912 |
| Blumeria hordei: K1 |
Hinze et al.74 |
NCBI Taxonomy ID: 1283759 |
| Rhizophagus irregularis: DAOM197198 |
Symplanta |
00101SP; https://www.symplanta.com/productcatalog.html |
| Barley: control: Hordeum vulgare (cv. Golden Promise Fast) |
Gol et al.42 |
N/A |
| Barley: gbp1 gbp2 #1: Hordeum vulgare (cv. Golden Promise Fast) gbp1-1 gbp2-1 |
this paper |
N/A |
| Barley: gbp1 gbp2 #2: Hordeum vulgare (cv. Golden Promise Fast) gbp1-2 gbp2-2/gbp2-3 |
this paper |
N/A |
| Nicotiana benthamiana |
The Sainsbury Laboratory, United Kingdom (Prof. Dr. Cyril Zipfel) |
N/A |
| Oligonucleotides |
| Primers for cloning see Table S1 |
this paper |
N/A |
| Primers for site-directed mutagenesis see Table S1 |
this paper |
N/A |
| Primers for CRISPR/Cas9 gRNA see Table S1 |
this paper |
N/A |
| Primers for S. indica colonization assay (qPCR) see Table S1 |
Chandrasekar et al.33 |
N/A |
| Primers for S. vermifera colonization assay (qPCR) see Table S1 |
Mahdi et al.75 |
N/A |
| Primers for B. sorokiniana colonization assay (qPCR) see Table S1 |
Mahdi et al.75 |
N/A |
| Primers for H. vulgare colonization assay (qPCR) see Table S1 |
Deshmukh et al.76 |
Accession no. M60175 |
| Primers for barley PR10 expression (qPCR) see Table S1 |
Schäfer et al.77 |
N/A |
| Recombinant DNA |
| pXCScpmv-HAStrep |
Witte et al.78; Myrach et al.79 |
N/A |
| pXCScpmv-GBP1-HAStrep |
This paper |
N/A |
| pXCScpmv-GBP1_E500A-HAStrep |
This paper |
N/A |
| pMGE625 |
Martin Luther University Halle-Wittenburg, Germany (Prof. Dr. Johannes Stuttmann) |
N/A |
| pMGE626 |
Martin Luther University Halle-Wittenburg, Germany (Prof. Dr. Johannes Stuttmann) |
N/A |
| pMGE628 |
Martin Luther University Halle-Wittenburg, Germany (Prof. Dr. Johannes Stuttmann) |
N/A |
| pMGE629 |
Martin Luther University Halle-Wittenburg, Germany (Prof. Dr. Johannes Stuttmann) |
N/A |
| pMGE599 |
Martin Luther University Halle-Wittenburg, Germany (Prof. Dr. Johannes Stuttmann) |
N/A |
| pMP202 |
Martin Luther University Halle-Wittenburg, Germany (Prof. Dr. Johannes Stuttmann) |
N/A |
| Software and algorithms |
| Flexanalysis software (v. 4.0) |
Bruker Daltonics |
https://www.bruker.com/en.html |
| CRISPOR web tool (v. 4.97) |
Concordet and Haeussler80 |
http://crispor.tefor.net/ |
| ImageJ (v. 1.53) |
Schneider et al.81 |
https://imagej.nih.gov/ij/ |
| SparkControl (v. 2.1) |
Tecan |
https://lifesciences.tecan.com/multimode-plate-reader?p=tab--3 |
| Scaffold (v. 4) |
Proteome Software |
https://www.proteomesoftware.com/products/scaffold-5 |
| MSConvert package |
ProteoWizard |
https://proteowizard.sourceforge.io/download.html |
| Mascot server (v. 2.3) |
Matrix Science |
https://www.matrixscience.com/server.html |
| Other |
| TECAN SPARK 10 M multiwell plate reader |
Tecan |
N/A |
| PD MidiTrap G-10 column |
Cytiva |
28918011 |
| TCS SP8 confocal microscope |
Leica |
https://www.leica-microsystems.com/ |
| AxioStar light microscope |
Carl Zeiss |
N/A |
| Silica gel 60 F254 aluminum TLC plate |
Merck Millipore |
105554 |
| MALDI-TOF mass spectrometer (rapifleX instrument) |
Bruker |
https://www.bruker.com/en/products-and-solutions/mass-spectrometry/maldi-tof/rapiflex.html |
| Reverse phase trap column (Acclaim PepMap, C18 5 μm, 100 μm x 2 cm) |
Thermo Scientific |
N/A |
| Analytical column (Acclaim PepMap 100, C18 3 μm, 75 μm x 50 cm) |
Thermo Scientific |
CAT#164570 |
| Orbitrap Fusion Tribrid mass spectrometer |
Thermo Scientific |
https://www.thermofisher.com/de/de/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-tribrid-mass-spectrometers.html#fusion-tribrid |
| Nanoflow-UHPLC system (UltiMate3000 Dionex) |
Thermo Scientific |
N/A |
| PD-10 desalting column |
Sigma Aldrich |
GE17-0851-01 |