diff --git a/assays/BarleyPhenotyping/protocols/BarleyMaterialAndGrowthConditions.md b/assays/BarleyPhenotyping/protocols/BarleyMaterialAndGrowthConditions.md index dbbef4324aec847d7b3d01f845b4234fb28c65a0..fd3f125e37ecf0191196cc714fe9108e0ce7dbd3 100644 --- a/assays/BarleyPhenotyping/protocols/BarleyMaterialAndGrowthConditions.md +++ b/assays/BarleyPhenotyping/protocols/BarleyMaterialAndGrowthConditions.md @@ -4,7 +4,7 @@ All experiments, including the generation of CRISPR/Cas9 knock-out lines, were p ## Barley growth conditions (6 days old) -Seeds were germinated on wet filter paper at room temperature in the dark under sterile conditions for three days before transfer to sterile jars containing solid 1/10 plant nutrition medium (PNM), pH 5.7 and 0.4% Gelrite (Duchefa, Haarlem, the Netherlands). Seedlings were cultured for six days in a growth chamber under long-day conditions (day/night cycle of 16/8 h, 22 C/18 C, light intensity of 108 mmol$m2$s1). Root and shoot fresh weight as well as length of the barley plants were measured. +Seeds were germinated on wet filter paper at room temperature in the dark under sterile conditions for three days before transfer to sterile jars containing solid 1/10 plant nutrition medium (PNM), pH 5.7 and 0.4% Gelrite (Duchefa, Haarlem, the Netherlands). Seedlings were cultured for six days in a growth chamber under long-day conditions (day/night cycle of 16/8 h, 22 C/18 C, light intensity of 108 mmol m-2 s-1). Root and shoot fresh weight as well as length of the barley plants were measured. ## Barley growth conditions (28 days old)