From 2cd49f0904ca27b485d5f92f3299a56e741728a7 Mon Sep 17 00:00:00 2001
From: Pia Saake <psaake@uni-koeln.de>
Date: Tue, 15 Oct 2024 09:30:38 +0200
Subject: [PATCH] protocol

---
 .../protocols/BarleyMaterialAndGrowthConditions.md              | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/assays/BarleyPhenotyping/protocols/BarleyMaterialAndGrowthConditions.md b/assays/BarleyPhenotyping/protocols/BarleyMaterialAndGrowthConditions.md
index dbbef43..fd3f125 100644
--- a/assays/BarleyPhenotyping/protocols/BarleyMaterialAndGrowthConditions.md
+++ b/assays/BarleyPhenotyping/protocols/BarleyMaterialAndGrowthConditions.md
@@ -4,7 +4,7 @@ All experiments, including the generation of CRISPR/Cas9 knock-out lines, were p
 
 ## Barley growth conditions (6 days old) 
 
-Seeds were germinated on wet filter paper at room temperature in the dark under sterile conditions for three days before transfer to sterile jars containing solid 1/10 plant nutrition medium (PNM), pH 5.7 and 0.4% Gelrite (Duchefa, Haarlem, the Netherlands). Seedlings were cultured for six days in a growth chamber under long-day conditions (day/night cycle of 16/8 h, 22 C/18 C, light intensity of 108 mmol$m2$s1). Root and shoot fresh weight as well as length of the barley plants were measured. 
+Seeds were germinated on wet filter paper at room temperature in the dark under sterile conditions for three days before transfer to sterile jars containing solid 1/10 plant nutrition medium (PNM), pH 5.7 and 0.4% Gelrite (Duchefa, Haarlem, the Netherlands). Seedlings were cultured for six days in a growth chamber under long-day conditions (day/night cycle of 16/8 h, 22 C/18 C, light intensity of 108 mmol m-2 s-1). Root and shoot fresh weight as well as length of the barley plants were measured. 
 
 ## Barley growth conditions (28 days old) 
 
-- 
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