From 2df2092a8abd4546b8f787ae25983e4ee34d1b92 Mon Sep 17 00:00:00 2001
From: Viktoria Petrova <vipet103@hhu.de>
Date: Sat, 13 Jan 2024 12:57:42 +0100
Subject: [PATCH] add Biotynilation of laminarin protocol

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 assays/ProteinPull-down/protocols/BiotinylationOfLaminarin.md | 2 ++
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 create mode 100644 assays/ProteinPull-down/protocols/BiotinylationOfLaminarin.md

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+## Biotinylation of laminarin
+Laminarin (final concentration ∼ 60 mM) was incubated with biotin-hydrazide (120 mM) and sodium cyanoborohydride (1 M) for 2 h at 65 °C. The product was purified on a PD MidiTrap G-10 column (GE Healthcare) according to the manufacturer’s description. Success of biotinylation was validated via mass spectrometry. The immunogenic capacity of biotinylated laminarin was confirmed via ROS burst assays.
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